Protein competitive adsorption and its effect on cell adhesion
文献类型：会议论文
| 作者 | Ying PQ; Jin G(靳刚) ; Tao ZL
|
| 出版日期 | 2000 |
| 会议名称 | IEEE-EMBS Asia-Pacific Conference on Biomedical Engineering |
| 会议日期 | SEP 26-28, 2000 |
| 会议地点 | HANGZHOU, PEOPLES R CHINA |
| 通讯作者 | Ying, PQ (reprint author), Chinese Acad Sci, Inst Mech, Beijing 100080, Peoples R China. |
| 中文摘要 | The purpose of this research was to investigate the competitive adsorption of bovine serum albumin (BSA) and collagen on various hydrophilicity surfaces with Imaging Ellipsometry and its effects on cell adhesion. Silicon surfaces with various hydrophilicity were incubated with the binary protein solution and an anti-BSA solution in order. By measuring the thickness distribution of the protein layers with Imaging Ellipsometry, protein competitive adsorption on substrata could be deduced. Substrata coated with protein were incubated in NIH3T3 fibroblasts or ROS 17/2.8 cells suspension The results showed that (1) in the binary protein solution of BSA and collagen, BSA preferentially adsorbed onto hydrophobic surfaces, but collagen on hydrophilic surfaces. (2) NIH3T3 fibroblasts adhered mostly and spread well on hydrophilic surface but hardly spread on hydrophobic surfaces conditioned with dimethyldichlorosilane (DMS). But it was reversed for ROS cell adhesion on hydrophobic surfaces conditioned with octadecyltrichlorosilane (OTS) and hydrophilic surfaces showing that cell adhesion was influenced by not only protein competitive adsorption but also cell type. |
| 收录类别 | CPCI-S |
| 会议录 | IEEE-EMBS ASIA PACIFIC CONFERENCE ON BIOMEDICAL ENGINEERING - PROCEEDINGS, PTS 1 & 2
 |
| 会议录出版者 | WORLD PUBLISHING CORPORATION |
| 会议录出版地 | BEIJING ; 137 CHAONEI DAJIE, BEIJING 100010, PEOPLES R CHINA |
| 语种 | 英语 |
| ISBN号 | 7-5062-4828-X |
| WOS记录号 | WOS:000165806500270 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/58960]  |
| 专题 | 力学研究所_力学所知识产出(1956-2008) |
| 推荐引用方式 GB/T 7714 | Ying PQ,Jin G,Tao ZL. Protein competitive adsorption and its effect on cell adhesion[C]. 见:IEEE-EMBS Asia-Pacific Conference on Biomedical Engineering. HANGZHOU, PEOPLES R CHINA. SEP 26-28, 2000. |
入库方式： OAI收割
来源：力学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。