Crystal structure and site-directed mutagenesis of a nitroalkane oxidase from Streptomyces ansochromogenes
文献类型：期刊论文
| 作者 | Li YH(李艳华) ; Gao ZQ(高增强); Hou HF(侯海峰); Dong YH(董宇辉); Li, YH; Gao, ZQ; Hou, HF; Li, L; Zhang, JH; Yang, HH
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| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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| 出版日期 | 2011 |
| 卷号 | 405期号:3页码:344-348 |
| 关键词 | Nitroalkane oxidase Crystallization Site-directed mutagenesis TIM barrel |
| 通讯作者 | [Li, Yanhua ; Gao, Zengqiang ; Hou, Haifeng ; Dong, Yuhui] Chinese Acad Sci, Inst High Energy Phys, Beijing Synchrotron Radiat Facil, Beijing 100049, Peoples R China ; [Li, Yanhua ; Li, Lei ; Zhang, Jihui ; Yang, Haihua ; Tan, Huarong] Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China |
| 英文摘要 | Nitroalkane oxidase (NAO) catalyzes neutral nitroalkanes to their corresponding aldehydes or ketones, hydrogen peroxide and nitrite. The crystal structure of NAO from Streptomyces ansochromogenes was determined; it consists of two domains, a TIM barrel domain bound to FMN and C-terminal domain with a novel folding pattern. Site-directed mutagenesis of His(179), which is spatially adjacent to FMN, resulted in the loss of enzyme activity, demonstrating that this amino acid residue is important for catalysis. The crystal structure of mutant H179D-nitroethane was also analyzed. Interestingly, Sa-NAO shows the typical function as nitroalkane oxidase but its structure is similar to that of 2-nitropropane dioxygenase. Overall, these results suggest that Sa-NAO is a novel nitroalkane oxidase with TIM barrel structure. (C) 2010 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 原文出处 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000287996500003 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/237073]  |
| 专题 | 高能物理研究所_多学科研究中心 高能物理研究所_粒子天体物理中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Li YH,Gao ZQ,Hou HF,et al. Crystal structure and site-directed mutagenesis of a nitroalkane oxidase from Streptomyces ansochromogenes[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2011,405(3):344-348. |
| APA | 李艳华.,高增强.,侯海峰.,董宇辉.,Li, YH.,...&Tan, HR.(2011).Crystal structure and site-directed mutagenesis of a nitroalkane oxidase from Streptomyces ansochromogenes.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,405(3),344-348. |
| MLA | 李艳华,et al."Crystal structure and site-directed mutagenesis of a nitroalkane oxidase from Streptomyces ansochromogenes".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 405.3(2011):344-348. |
入库方式： OAI收割
来源：高能物理研究所
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