Light flux density threshold at which protein denaturation is induced by synchrotron radiation circular dichroism beamlines
文献类型：期刊论文
| 作者 | Miles, AJ; Janes, RW; Brown, A; Clarke, DT; Sutherland, JC; Tao, Y ; Wallace, BA; Hoffmann, SV; Tao Y(陶冶)
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| 刊名 | JOURNAL OF SYNCHROTRON RADIATION
 |
| 出版日期 | 2008 |
| 卷号 | 15期号:4页码:420-422 |
| 关键词 | protein denaturation radiation damage synchrotron radiation circular dichroism spectroscopy photon flux vacuum ultraviolet wavelengths |
| 通讯作者 | [Hoffmann, S. V.] Univ Aarhus, Inst Storage Ring Facil, DK-8000 Aarhus C, Denmark ; [Miles, A. J. ; Wallace, B. A.] Univ London, Birkbeck Coll, Dept Crystallog, London WC1E 7HX, England ; [Janes, Robert W.] Univ London, Sch Biol & Chem Sci, London E1 4NS, England ; [Brown, A. ; Clarke, D. T.] STFC, Daresbury Lab, Warrington WA4 4AD, Cheshire, England ; [Sutherland, J. C.] Brookhaven Natl Lab, Dept Biol, Upton, NY 11973 USA ; [Sutherland, J. C.] E Carolina Univ, Dept Phys, Greenville, NC 27858 USA ; [Tao, Y.] Chinese Acad Sci, Inst High Energy Phys, Beijing Synchrotron Radiat Facil, Beijing, Peoples R China |
| 英文摘要 | New high-flux synchrotron radiation circular dichroism (SRCD) beamlines are providing important information for structural biology, but can potentially cause denaturation of the protein samples under investigation. This effect has been studied at the new CD1 dedicated SRCD beamline at ISA in Denmark, where radiation-induced thermal damage effects were observed, depending not only on the radiation flux but also on the focal spot size of the light. Comparisons with similar studies at other SRCD facilities worldwide has lead to the estimation of a flux density threshold under which SRCD beamlines should be operated when samples are to be exposed to low-wavelength vacuum ultraviolet radiation for extended periods of time. |
| 学科主题 | Instruments & Instrumentation; Optics; Physics |
| 类目[WOS] | Instruments & Instrumentation ; Optics ; Physics, Applied |
| 研究领域[WOS] | Instruments & Instrumentation ; Optics ; Physics |
| 原文出处 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000256720900016 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/237408]  |
| 专题 | 高能物理研究所_多学科研究中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Miles, AJ,Janes, RW,Brown, A,et al. Light flux density threshold at which protein denaturation is induced by synchrotron radiation circular dichroism beamlines[J]. JOURNAL OF SYNCHROTRON RADIATION,2008,15(4):420-422. |
| APA | Miles, AJ.,Janes, RW.,Brown, A.,Clarke, DT.,Sutherland, JC.,...&陶冶.(2008).Light flux density threshold at which protein denaturation is induced by synchrotron radiation circular dichroism beamlines.JOURNAL OF SYNCHROTRON RADIATION,15(4),420-422. |
| MLA | Miles, AJ,et al."Light flux density threshold at which protein denaturation is induced by synchrotron radiation circular dichroism beamlines".JOURNAL OF SYNCHROTRON RADIATION 15.4(2008):420-422. |
入库方式： OAI收割
来源：高能物理研究所
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