Quantitative investigation of two metallohydrolases by X-ray absorption spectroscopy near-edge spectroscopy
文献类型：期刊论文
| 作者 | Zhao, W ; Chu WS(储旺盛); Yang FF(杨飞飞); Yu MJ(于梅娟); Chen DL(陈栋梁); Guo XY(郭兴运); Zhou DW(周大卫); Wu ZY(吴自玉); Chu, WS; Yang, FF
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| 刊名 | NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT
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| 出版日期 | 2007 |
| 卷号 | 580期号:1页码:451-456 |
| 关键词 | BioXAS MXAN XANES metalloprotein LiPDF acutolysiu-C |
| 通讯作者 | Chinese Acad Sci, Inst High Energy Phys, Beijing 100049, Peoples R China ; Chinese Acad Sci, Inst High Energy Phys, Beijing 100049, Peoples R China ; Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Anhui, Peoples R China ; Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Anhui, Peoples R China ; Ist Nazl Fis Nucl, Lab Nazl Frascati, I-00044 Frascati, Italy ; Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China |
| 英文摘要 | The last several years have witnessed a tremendous increase in biological applications using X-ray absorption spectroscopy (BioXAS), thanks to continuous advancements in synchrotron radiation (SR) sources and detector technology. However, XAS applications in many biological systems have been limited by the intrinsic limitations of the Extended X-ray Absorption Fine Structure (EXAFS) technique e.g., the lack of sensitivity to bond angles. As a consequence, the application of the X-ray absorption near-edge structure (XANES) spectroscopy changed this scenario that is now continuously changing with the introduction of the first quantitative XANES packages such as Minut XANES (MXAN). Here we present and discuss the XANES code MXAN, a novel XANES-fitting package that allows a quantitative analysis of experimental data applied to Zn K-edge spectra of two metalloproteins: Leptospira interrogans Peptide deformylase (LiPDF) and acutolysin-C, a representative of snake venom metalloproteinases (SVMPs) from Agkistrodon acutus venom. The analysis on these two metallohydrolases reveals that proteolytic activities are correlated to subtle conformation changes around the zinc ion. In particular, this quantitative study clarifies the occurrence of the LiPDF catalytic mechanism via a two-water-molecules model, whereas in the acutolysin-C we have observed a different proteolytic activity correlated to structural changes around the zinc ion induced by pH variations. (C) 2007 Elsevier B.V. All rights reserved. |
| 学科主题 | Instruments & Instrumentation; Nuclear Science & Technology; Physics; Spectroscopy |
| 类目[WOS] | Instruments & Instrumentation ; Nuclear Science & Technology ; Physics, Particles & Fields ; Spectroscopy |
| 研究领域[WOS] | Instruments & Instrumentation ; Nuclear Science & Technology ; Physics ; Spectroscopy |
| 原文出处 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000249741300117 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/237534]  |
| 专题 | 高能物理研究所_多学科研究中心 高能物理研究所_实验物理中心 高能物理研究所_粒子天体物理中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Zhao, W,Chu WS,Yang FF,et al. Quantitative investigation of two metallohydrolases by X-ray absorption spectroscopy near-edge spectroscopy[J]. NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT,2007,580(1):451-456. |
| APA | Zhao, W.,储旺盛.,杨飞飞.,于梅娟.,陈栋梁.,...&Wu, ZY.(2007).Quantitative investigation of two metallohydrolases by X-ray absorption spectroscopy near-edge spectroscopy.NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT,580(1),451-456. |
| MLA | Zhao, W,et al."Quantitative investigation of two metallohydrolases by X-ray absorption spectroscopy near-edge spectroscopy".NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT 580.1(2007):451-456. |
入库方式： OAI收割
来源：高能物理研究所
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