ATP-induced noncooperative thermal unfolding of hen lysozyme
文献类型：期刊论文
| 作者 | Liu, HL; Yin, PD; He, SN; Sun, ZH ; Tao, Y; Huang, Y; Zhuang, H; Zhang, GB; Wei, SQ; Tao Y(陶冶)
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| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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| 出版日期 | 2010 |
| 卷号 | 397期号:3页码:598-602 |
| 关键词 | Hen egg white lysozyme Synchrotron radiation circular dichroism Secondary structure Noncooperative unfolding Partially unfolded intermediate |
| 通讯作者 | [Liu, Honglin ; Yin, Peidong ; He, Shengnan ; Sun, Zhihu ; Zhang, Guobin ; Wei, Shiqiang] Univ Sci & Technol China, Natl Synchrotron Radiat Lab, Hefei 230029, Anhui, Peoples R China ; [Tao, Ye ; Huang, Yan ; Zhuang, Hao] Chinese Acad Sci, Inst High Energy Phys, Beijing 100049, Peoples R China |
| 英文摘要 | To understand the role of ATP underlying the enhanced amyloidosis of hen egg white lysozyme (HEWL), the synchrotron radiation circular dichroism, combined with tryptophan fluorescence, dynamic light-scattering, and differential scanning calorimetry, is used to examine the alterations of the conformation and thermal unfolding pathway of the HEWL in the presence of ATP, Mg(2+)-ATP, ADP, AMP, etc. It is revealed that the binding of ATP to HEWL through strong electrostatic interaction changes the secondary structures of HEWL and makes the exposed residue W62 move into hydrophobic environments. This alteration of W62 decreases the beta-domain stability of HEWL, induces a noncooperative unfolding of the secondary structures, and produces a partially unfolded intermediate. This intermediate containing relatively rich alpha-helix and less beta-sheet structures has a great tendency to aggregate. The results imply that the ease of aggregating of HEWL is related to the extent of denaturation of the amyloidogenic region, rather than the electrostatic neutralizing effect or monomeric beta-sheet enriched intermediate. (C) 2010 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 原文出处 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000279974600041 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/238071]  |
| 专题 | 高能物理研究所_多学科研究中心 高能物理研究所_计算中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Liu, HL,Yin, PD,He, SN,et al. ATP-induced noncooperative thermal unfolding of hen lysozyme[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2010,397(3):598-602. |
| APA | Liu, HL.,Yin, PD.,He, SN.,Sun, ZH.,Tao, Y.,...&庄浩.(2010).ATP-induced noncooperative thermal unfolding of hen lysozyme.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,397(3),598-602. |
| MLA | Liu, HL,et al."ATP-induced noncooperative thermal unfolding of hen lysozyme".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 397.3(2010):598-602. |
入库方式： OAI收割
来源：高能物理研究所
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