X-ray absorption near edge structure study on Acutolysin-C, a zinc-metalloproteinase from Agkistrodon acutus venom: Insight into the acid-inactive mechanism
文献类型：期刊论文
| 作者 | Zhao W(赵维); Chu WS(储旺盛) ; Li SJ(李树军); Gao B(高斌); Hu TD(胡天斗); Wu ZY(吴自玉); Zhao, W; Chu, WS; Li, SJ; Liu, YW
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| 刊名 | SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY
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| 出版日期 | 2007 |
| 卷号 | 62期号:11页码:1246-1251 |
| 关键词 | Acutolysin-C metalloproteinase BioXAS XANES MXAN |
| 通讯作者 | [Zhao, Wei ; Liu, Yiwei ; Niu, Liwen ; Teng, Maikun] Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Anhui, Peoples R China ; [Zhao, Wei ; Liu, Yiwei ; Niu, Liwen ; Teng, Maikun] Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Anhui, Peoples R China ; [Zhao, Wei ; Chu, Wangsheng ; Li, Shujun ; Gao, Bin ; Hu, Tiandou ; Wu, Ziyu] Chinese Acad Sci, Inst High Energy Phys, Beijing Synchrotron Radiat Facil, Beijing 100049, Peoples R China ; [Benfatto, Maurizio ; Wu, Ziyu] Ist Nazl Fis Nucl, Lab Nazl Frascati, I-00044 Frascati, Italy |
| 英文摘要 | Acutolysin-C, a snake-venom zinc metalloproteinase, displays a distinct pH-dependent proteolytic activity, which has been tentatively assigned to a structural change of the zinc-containing catalytic center. In this work we compare X-ray absorption near-edge structure (XANES) experimental spectra at the Zn K-edge and theoretical calculations of solutions at different pH values. The experimental data show clear differences confirmed by a best fit using the MXAN procedure. The results show that, when pH decreases from pH 8.0 to pH 3.0, the zinc-coordinating, catalytic water molecule moves far from the Glu143 residue that is considered to play an essential role in the proteolytic process. Data suggests that this is the possible mechanism that deactivates the metalloproteinase. (C) 2007 Published by Elsevier B.V. |
| 学科主题 | Spectroscopy |
| 类目[WOS] | Spectroscopy |
| 研究领域[WOS] | Spectroscopy |
| 原文出处 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000251943100010 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/239222]  |
| 专题 | 高能物理研究所_多学科研究中心 高能物理研究所_实验物理中心 高能物理研究所_粒子天体物理中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Zhao W,Chu WS,Li SJ,et al. X-ray absorption near edge structure study on Acutolysin-C, a zinc-metalloproteinase from Agkistrodon acutus venom: Insight into the acid-inactive mechanism[J]. SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY,2007,62(11):1246-1251. |
| APA | 赵维.,储旺盛.,李树军.,高斌.,胡天斗.,...&Wu, ZY.(2007).X-ray absorption near edge structure study on Acutolysin-C, a zinc-metalloproteinase from Agkistrodon acutus venom: Insight into the acid-inactive mechanism.SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY,62(11),1246-1251. |
| MLA | 赵维,et al."X-ray absorption near edge structure study on Acutolysin-C, a zinc-metalloproteinase from Agkistrodon acutus venom: Insight into the acid-inactive mechanism".SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY 62.11(2007):1246-1251. |
入库方式： OAI收割
来源：高能物理研究所
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