The inhibition of death receptor mediated apoptosis through lysosome stabilization following internalization of carboxyfullerene nanoparticles
文献类型：期刊论文
| 作者 | Li W(李炜) ; Zhao LN(赵丽娜); Zhao YL(赵宇亮); Chen CY(陈春英); Li, W; Zhao, LN; Wei, TT; Zhao, YL; Chen, CY
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| 刊名 | BIOMATERIALS
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| 出版日期 | 2011 |
| 卷号 | 32期号:16页码:4030-4041 |
| 关键词 | Fullerene derivative Endocytosis Lysosomal membrane Apoptosis Hsp 70 |
| 通讯作者 | [Chen, Chunying] Natl Ctr Nanosci & Technol China, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China ; [Li, Wei ; Zhao, Lina ; Zhao, Yuliang ; Chen, Chunying] Chinese Acad Sci, Inst High Energy Phys, CAS Key Lab Biomed Effects Nanomat & Nanosaftey, Beijing 100049, Peoples R China ; [Li, Wei ; Zhao, Lina ; Zhao, Yuliang ; Chen, Chunying] Chinese Acad Sci, Natl Ctr Nanosci & Technol, Beijing 100049, Peoples R China ; [Wei, Taotao] Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China |
| 英文摘要 | Cells undergo apoptosis through two major pathways, the extrinsic pathway (death receptor pathway) and the intrinsic pathway (the mitochondrial pathway). It is well known that nanomaterials of water-soluble fullerene derivatives are potent antioxidants and help to prevent the overproduction of mitochondrial reactive oxygen species (ROS). However, whether their interaction with cells via the death receptor pathway is direct or indirect remains poorly understood. Here, we show that a bis-adduct malonic acid derivative of fullerene, C(60)(C(COOH)(2))(2), inhibits tumor necrosis factor alpha-initiated cellular apoptosis via stabilizing lysosomes. Data presented here demonstrate that nano-sized aggregates of this water-soluble fullerene derivative are endocytosed into cells and enriched in the lysosomes. During the internalization of C(60)(C(COOH)(2))(2), the expression of Hsp 70 is significantly upregulated, promoting cell survival by inhibiting the permeabilization of lysosomal membranes. In addition, the acidic environment inside lysosomes has a marked but temporary effect on the size distribution of fullerenic nanoparticles, and may disperse the aggregated C(60)(C(COOH)(2))(2) nanoparticles into single molecules or smaller aggregates. These single molecules or smaller aggregates may insert into the lysosomal membranes, further stabilizing them and decreasing the release of cathepsins from lysosomes, leading to the inhibition of tumor necrosis factor-induced apoptosis. C(60)(C(COOH)(2))(2) nanoparticles can thus protect cells by stabilizing lysosomal membranes via both upregulated expression of Hsp 70 and by their interactions with lysosomal membranes. (C) 2011 Elsevier Ltd. All rights reserved. |
| 学科主题 | Engineering; Materials Science |
| 类目[WOS] | Engineering, Biomedical ; Materials Science, Biomaterials |
| 研究领域[WOS] | Engineering ; Materials Science |
| 原文出处 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000290196700016 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/239956]  |
| 专题 | 高能物理研究所_多学科研究中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Li W,Zhao LN,Zhao YL,et al. The inhibition of death receptor mediated apoptosis through lysosome stabilization following internalization of carboxyfullerene nanoparticles[J]. BIOMATERIALS,2011,32(16):4030-4041. |
| APA | 李炜.,赵丽娜.,赵宇亮.,陈春英.,Li, W.,...&Chen, CY.(2011).The inhibition of death receptor mediated apoptosis through lysosome stabilization following internalization of carboxyfullerene nanoparticles.BIOMATERIALS,32(16),4030-4041. |
| MLA | 李炜,et al."The inhibition of death receptor mediated apoptosis through lysosome stabilization following internalization of carboxyfullerene nanoparticles".BIOMATERIALS 32.16(2011):4030-4041. |
入库方式： OAI收割
来源：高能物理研究所
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