Surface chemistry and aspect ratio mediated cellular uptake of Au nanorods
文献类型：期刊论文
| 作者 | Qiu, Y; Liu, Y ; Wang, LM; Xu, LG; Bai, R; Ji, YL; Wu, XC; Zhao, YL; Li, YF; Chen, CY
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| 刊名 | BIOMATERIALS
 |
| 出版日期 | 2010 |
| 卷号 | 31期号:30页码:7606-7619 |
| 关键词 | Gold Nanoparticles Nanorods Cytotoxicity Aspect ratio Surface modification |
| 通讯作者 | [Qiu, Yang ; Liu, Ying ; Wang, Liming ; Xu, Ligeng ; Bai, Ru ; Ji, Yinglu ; Zhao, Yuliang ; Chen, Chunying] Natl Ctr Nanosci & Technol China, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China ; [Zhao, Yuliang ; Li, Yufeng ; Chen, Chunying] Chinese Acad Sci, Inst High Energy Phys, Beijing 100049, Peoples R China ; [Ji, Yinglu ; Wu, Xiaochun] Natl Ctr Nanosci & Technol China, CAS Key Lab Standardizat & Measurement Nanotechno, Beijing 100190, Peoples R China |
| 英文摘要 | Gold nanorods (Au NRs) have been recognized as promising materials for biomedical applications, like sensing, imaging, gene and drug delivery and therapy, but their toxicological issues are still controversial, especially for the Au NRs synthesized with seed-mediated method. In this study, we investigated the influence of aspect ratio and surface coating on their toxicity and cellular uptake. The cellular uptake is highly dependent on the aspect ratio and surface coating. However, the surface chemistry has the dominant roles since PDDAC-coated Au NRs exhibit a much greater ability to be internalized by the cells. The present data demonstrated shape-independent but coating-dependent cytotoxicity. Both the CTAB molecules left in the suspended solution and on the surface of Au NRs were identified as the actual cause of cytotoxicity. CTAB can enter cells with or without Au NRs, damage mitochondria, and then induce apoptosis. The effects of surface coating upon toxicity and cellular uptake were also examined using Au NRs with different coatings. When Au NRs were added into the medium, the proteins were quickly adsorbed onto the Au NRs that made the surface negatively charged. The surface charge may not directly affect the cellular uptake. We further demonstrated that the amount of serum proteins, especially for BSA, adsorbed on the Au NRs had a positive correlation with the capacity of Au NRs to enter cells. In addition, we have successfully revealed that the cationic PDDAC-coated Au NRs with an aspect ratio of 4 possess an ideal combination of both negligible toxicity and high cellular uptake efficiency, showing a great promise as photothermal therapeutic agents. (C) 2010 Elsevier Ltd. All rights reserved. |
| 学科主题 | Engineering; Materials Science |
| 类目[WOS] | Engineering, Biomedical ; Materials Science, Biomaterials |
| 研究领域[WOS] | Engineering ; Materials Science |
| 原文出处 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000281498600002 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/240126]  |
| 专题 | 高能物理研究所_多学科研究中心 高能物理研究所_实验物理中心 高能物理研究所_加速器中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Qiu, Y,Liu, Y,Wang, LM,et al. Surface chemistry and aspect ratio mediated cellular uptake of Au nanorods[J]. BIOMATERIALS,2010,31(30):7606-7619. |
| APA | Qiu, Y.,Liu, Y.,Wang, LM.,Xu, LG.,Bai, R.,...&陈春英.(2010).Surface chemistry and aspect ratio mediated cellular uptake of Au nanorods.BIOMATERIALS,31(30),7606-7619. |
| MLA | Qiu, Y,et al."Surface chemistry and aspect ratio mediated cellular uptake of Au nanorods".BIOMATERIALS 31.30(2010):7606-7619. |
入库方式： OAI收割
来源：高能物理研究所
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