Rational design of a mononuclear metal site into the archaeal Rieske-type protein scaffold
文献类型：期刊论文
| 作者 | Iwasaki, T; Kounosu, A; Tao Y(陶冶) ; Tao, Y; Li, ZR; Shokes, JE; Cosper, NJ; Imai, T; Urushiyama, A; Scott, RA
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| 刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY
 |
| 出版日期 | 2005 |
| 卷号 | 280期号:10页码:9129-9134 |
| 通讯作者 | Nippon Med Coll, Dept Biochem & Mol Biol, Bunkyo Ku, Tokyo 1138602, Japan ; Nippon Med Coll, Dept Biochem & Mol Biol, Bunkyo Ku, Tokyo 1138602, Japan ; Chinese Acad Sci, Synchrotron Radiat Facil, Inst High Energy Phys, Beijing 100039, Peoples R China ; Rikkyo Univ, Dept Chem, Toshima Ku, Tokyo 1718501, Japan ; Univ Georgia, Dept Chem, Athens, GA 30602 USA |
| 英文摘要 | Proteins containing Rieske-type [2Fe-2S] clusters play essential functions in all three domains of life. We engineered the two histidine ligands to the Rieske-type [2Fe2S] cluster in the hyperthermophilic archaeal Rieske-type ferredoxin from Sulfolobus solfataricus to modify types and spacing of ligands and successfully converted the metal and cluster type at the redox-active site with a minimal structural change to a native Rieske-type protein scaffold. Spectroscopic analyses unambiguously established a rubredoxin-type mononuclear Fe3+/2+ center at the engineered local metal-binding site ( Zn2+ occupies the iron site depending on the expression conditions). These results show the importance of types and spacing of ligands in the in vivo cluster recognition/ insertion/ assembly in biological metallosulfur protein scaffolds. We suggest that early ligand substitution and displacement events at the local metal-binding site(s) might have primarily allowed the metal and cluster type conversion in ancestral redox protein modules, which greatly enhanced their capabilities of conducting a wide range of unique redox chemistry in biological electron transfer conduits, using a limited number of basic protein scaffolds. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 原文出处 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000227453100062 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/240157]  |
| 专题 | 高能物理研究所_多学科研究中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Iwasaki, T,Kounosu, A,Tao Y,et al. Rational design of a mononuclear metal site into the archaeal Rieske-type protein scaffold[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2005,280(10):9129-9134. |
| APA | Iwasaki, T.,Kounosu, A.,陶冶.,Tao, Y.,Li, ZR.,...&Scott, RA.(2005).Rational design of a mononuclear metal site into the archaeal Rieske-type protein scaffold.JOURNAL OF BIOLOGICAL CHEMISTRY,280(10),9129-9134. |
| MLA | Iwasaki, T,et al."Rational design of a mononuclear metal site into the archaeal Rieske-type protein scaffold".JOURNAL OF BIOLOGICAL CHEMISTRY 280.10(2005):9129-9134. |
入库方式： OAI收割
来源：高能物理研究所
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