Speciation and subcellular location of Se-containing proteins in human liver studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and hydride generation-atomic fluorescence spectrometric detection
文献类型：期刊论文
| 作者 | Chen CY(陈春英); Zhao JJ(赵九江); Zhang PQ(章佩群); Chai ZF(柴之芳) ; Chen, CY; Zhao, JJ; Zhang, PQ; Chai, ZF
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| 刊名 | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
 |
| 出版日期 | 2002 |
| 卷号 | 372期号:3页码:426-430 |
| 关键词 | Se-containing proteins gel electrophoresis speciation HG-AFS human liver |
| 通讯作者 | Acad Sinica, Inst High Energy Phys, Lab Nucl Analyt Tech, Beijing 100039, Peoples R China |
| 英文摘要 | Speciation of Se-containing proteins in the subcellular fractions of human liver was studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by hydride generation-atomic fluorescence spectrometric (HG-AFS) detection. It was found that about 24 kinds of Se-containing proteins existed in subcellular fractions of normal human liver. The molecular weights (MW) of the subunits were mostly in the range 20-30 kDa and 50-80 kDa. Major Se-containing protein fractions at 61 kDa and 21 kDa are probably selenoprotein P and glutathione peroxidase, respectively. The 54 kDa protein is probably a thioredoxin reductase, which is presented in nuclei, mitochondria, lysosome, microsome and cytosol. We noticed that the Se-containing protein with the lowest MW of 9.3 kDa only existed in lysosome. Most of the proteins have not been identified and would require further investigation to characterize them. The specific subcellular distributions of different Se-containing proteins suggest that they could play important biological roles in each organelle. |
| 学科主题 | Biochemistry & Molecular Biology; Chemistry |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 原文出处 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000176184900006 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/237434]  |
| 专题 | 高能物理研究所_院士 中国科学院高能物理研究所_中国散裂中子源 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Chen CY,Zhao JJ,Zhang PQ,et al. Speciation and subcellular location of Se-containing proteins in human liver studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and hydride generation-atomic fluorescence spectrometric detection[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2002,372(3):426-430. |
| APA | 陈春英.,赵九江.,章佩群.,柴之芳.,Chen, CY.,...&Chai, ZF.(2002).Speciation and subcellular location of Se-containing proteins in human liver studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and hydride generation-atomic fluorescence spectrometric detection.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,372(3),426-430. |
| MLA | 陈春英,et al."Speciation and subcellular location of Se-containing proteins in human liver studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and hydride generation-atomic fluorescence spectrometric detection".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 372.3(2002):426-430. |
入库方式： OAI收割
来源：高能物理研究所
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