Analysis of trace elements in protein bands with synchrotron radiation X-ray fluorescence after electrophoresis separation
文献类型：期刊论文
| 作者 | Dong YX(董元兴); Gao YX(高愈希) ; Chen CY(陈春英); Li B(李柏); Xing L(邢丽); Yu HW(喻宏伟); He W(何伟); Huang YY(黄宇营); Chai ZF(柴之芳); Dong, YX
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| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2006 |
| 卷号 | 34期号:4页码:443-446 |
| 关键词 | trace element speciation analysis synchrotron radiation X-ray fluorescence electrophoresis metalloprotein |
| 其他题名 | 用同步辐射X荧光定量测定电泳分离后蛋白条带内的微量元素 |
| 通讯作者 | Chinese Acad Sci, Inst High Energy Phys, Beijing 100049, Peoples R China ; Xinzhou Teachers Univ, Xinzhou 034000, Peoples R China |
| 英文摘要 | A procedure has been proposed for the determination of Fe, Cu and Zn in protein bands with synchrotron radiation X-ray fluorescence (SRXRF) analysis after isoelectric focusing (IEF) separation. Calibration standards were prepared by adding certain amounts of metal ions and free-metal proteins to electrophoresis gel. Human hemoglobin was separated with IEF, then Fe, Cu and Zn in protein bands were analyzed by SRXRF. The calibration curves can be obtained in a range of 0-8 mu g/g metals and linear relationship between dosage of metals and fluorescent intensity can be observed (r>0.99). The method provides detection limits of 2.43, 1.12 and 0.96 mu g/g for Fe, Cu and Zn, and recoveries of 90.4% and 115.7% for Fe and Zn, respectively. The combined technique of SRXRF and IEF have advantages of quantifying trace elements in proteins and identifying the proteins simultaneously. It can be employed for speciation analysis of trace elements in biological samples. |
| 学科主题 | Chemistry |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 原文出处 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000237325400002 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/237504]  |
| 专题 | 高能物理研究所_院士 高能物理研究所_实验物理中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Dong YX,Gao YX,Chen CY,et al. Analysis of trace elements in protein bands with synchrotron radiation X-ray fluorescence after electrophoresis separation[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2006,34(4):443-446. |
| APA | 董元兴.,高愈希.,陈春英.,李柏.,邢丽.,...&Chai, ZF.(2006).Analysis of trace elements in protein bands with synchrotron radiation X-ray fluorescence after electrophoresis separation.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,34(4),443-446. |
| MLA | 董元兴,et al."Analysis of trace elements in protein bands with synchrotron radiation X-ray fluorescence after electrophoresis separation".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 34.4(2006):443-446. |
入库方式： OAI收割
来源：高能物理研究所
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