Combination of synchrotron radiation X-ray fluorescence with isoelectric focusing for study of metalloprotein distribution in cytosol of hepatocellular carcinoma and surrounding normal tissues
文献类型：期刊论文
| 作者 | Gao YX(高愈希) ; Liu yingbin; Chen CY(陈春英); Li B(李柏); He W(何伟); Huang YY(黄宇营); Chai ZF(柴之芳); Gao, YX; Liu, YB; Chen, CY
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| 刊名 | JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
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| 出版日期 | 2005 |
| 卷号 | 20期号:5页码:473-475 |
| 通讯作者 | Chinese Acad Sci, Inst High Energy Phys, Beijing 100039, Peoples R China ; Zhejiang Univ, Affiliated Hosp 2, Dept Surg, Hangzhou 310009, Peoples R China |
| 英文摘要 | The combined use of synchrotron radiation X-ray fluorescence (SRXRF) and isoelectric focusing (IEF) has been developed that allows comparison of the distribution of metal-containing proteins in cytosol of human hepatocellular carcinoma (HCC) with its surrounding "normal'' tissues. Proteins in samples from two individuals were separated with thin layer IEF, and the contents of Fe, Cu and Zn in protein bands were determined with SRXRF after drying the IEF gel. The results show that the metal-containing proteins in these samples of hepatocellular cytosol are specific for a given patient, and the distribution patterns of metal-containing proteins are distinguishable between the HCC and "normal'' tissues. The technique has the advantages of quantifying trace elements and identifying proteins simultaneously. |
| 学科主题 | Chemistry; Spectroscopy |
| 类目[WOS] | Chemistry, Analytical ; Spectroscopy |
| 研究领域[WOS] | Chemistry ; Spectroscopy |
| 原文出处 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000228689500015 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/237630]  |
| 专题 | 高能物理研究所_院士 高能物理研究所_实验物理中心 高能物理研究所_多学科研究中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Gao YX,Liu yingbin,Chen CY,et al. Combination of synchrotron radiation X-ray fluorescence with isoelectric focusing for study of metalloprotein distribution in cytosol of hepatocellular carcinoma and surrounding normal tissues[J]. JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY,2005,20(5):473-475. |
| APA | 高愈希.,Liu yingbin.,陈春英.,李柏.,何伟.,...&Chai, ZF.(2005).Combination of synchrotron radiation X-ray fluorescence with isoelectric focusing for study of metalloprotein distribution in cytosol of hepatocellular carcinoma and surrounding normal tissues.JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY,20(5),473-475. |
| MLA | 高愈希,et al."Combination of synchrotron radiation X-ray fluorescence with isoelectric focusing for study of metalloprotein distribution in cytosol of hepatocellular carcinoma and surrounding normal tissues".JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY 20.5(2005):473-475. |
入库方式： OAI收割
来源：高能物理研究所
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