Direct measurement of lanthanum uptake and distribution in internodal cells of Chara
文献类型：期刊论文
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| 作者 | Li ZJ(李子杰) ; Zhang ZY(张智勇); Jiang WJ(姜文君); Zhao YL(赵宇亮); Chai ZF(柴之芳); Li, ZJ; Zhang, ZY; Jiang, WJ; Yu, M; Zhou, YL
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| 刊名 | PLANT SCIENCE
 ; PLANT SCIENCE
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| 出版日期 | 2008 ; 2008 |
| 卷号 | 174期号:5页码:496-501 |
| 关键词 | Chara corallina distribution ICP-MS lanthanum uptake Chara corallina distribution ICP-MS lanthanum uptake |
| DOI | 10.1016/j.plantsci.2008.01.013 |
| 通讯作者 | [Li, Zijie ; Zhang, Zhiyong ; Jiang, Wenjun ; Zhao, Yuliang ; Chai, Zhifang] Chinese Acad Sci, Inst High Energy Phys, Key Lab Nucl Analyt Tech, Beijing 100049, Peoples R China ; [Li, Zijie ; Jiang, Wenjun] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China ; [Yu, Ming ; Zhou, Yunlong] Beijing Normal Univ, Sch Life Sci, Beijing 100875, Peoples R China ; [Li, Zijie ; Zhang, Zhiyong ; Jiang, Wenjun ; Zhao, Yuliang ; Chai, Zhifang] Chinese Acad Sci, Inst High Energy Phys, Key Lab Nucl Analyt Tech, Beijing 100049, Peoples R China ; [Li, Zijie ; Jiang, Wenjun] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China ; [Yu, Ming ; Zhou, Yunlong] Beijing Normal Univ, Sch Life Sci, Beijing 100875, Peoples R China |
| 文献子类 | Article |
| 英文摘要 | Rare earth elements (REEs) influence many physiological processes in plants, but the mechanism is still unknown. The question of whether REEs can enter plant cells is crucial in assessing the biological effects of REEs. In this paper, a method for determination of REE lanthanum (La) in ultratrace level in cell contents of giant algal cells was established. The uptake and subcellular distribution of La in internodal cells of Chara corallina were investigated. After incubation with 6 mu M LaCl3 for 72 h, about 0.523% of total lanthanum in the whole cell was internalized. Approximately 55% of the total intracellular lanthanum was in the cytoplasm, and lanthanum concentration in the cytoplasm was much higher than that in the vacuole. The concentration dependence of La3+ uptake occurred in a biphasic manner, which was tentatively attributed to the operation of two separate transport systems, a high-affinity system which is saturated at 10 mu M and a low affinity system which showed a linear dependence on concentration up to at least 100 mu M. Only the high-affinity system was studied in detail. Effects of channel activator, blocker and metabolic inhibition on lanthanum uptake through this system were investigated. The results suggest that lanthanum transport across plasma membrane was a metabolically mediated process and membrane potential difference was independent of lanthanum uptake. (C) 2008 Elsevier Ireland Ltd. All rights reserved.; Rare earth elements (REEs) influence many physiological processes in plants, but the mechanism is still unknown. The question of whether REEs can enter plant cells is crucial in assessing the biological effects of REEs. In this paper, a method for determination of REE lanthanum (La) in ultratrace level in cell contents of giant algal cells was established. The uptake and subcellular distribution of La in internodal cells of Chara corallina were investigated. After incubation with 6 mu M LaCl3 for 72 h, about 0.523% of total lanthanum in the whole cell was internalized. Approximately 55% of the total intracellular lanthanum was in the cytoplasm, and lanthanum concentration in the cytoplasm was much higher than that in the vacuole. The concentration dependence of La3+ uptake occurred in a biphasic manner, which was tentatively attributed to the operation of two separate transport systems, a high-affinity system which is saturated at 10 mu M and a low affinity system which showed a linear dependence on concentration up to at least 100 mu M. Only the high-affinity system was studied in detail. Effects of channel activator, blocker and metabolic inhibition on lanthanum uptake through this system were investigated. The results suggest that lanthanum transport across plasma membrane was a metabolically mediated process and membrane potential difference was independent of lanthanum uptake. (C) 2008 Elsevier Ireland Ltd. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Plant Sciences ; Biochemistry & Molecular Biology; Plant Sciences |
| 类目[WOS] | Biochemistry & Molecular Biology ; Plant Sciences |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Plant Sciences |
| URL标识 | 查看原文 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| 原文出处 | SCI |
| 语种 | 英语 ; 英语 |
| WOS记录号 | WOS:000255313800002 ; WOS:000255313800002 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/237716]  |
| 专题 | 高能物理研究所_院士 高能物理研究所_多学科研究中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Li ZJ,Zhang ZY,Jiang WJ,et al. Direct measurement of lanthanum uptake and distribution in internodal cells of Chara, Direct measurement of lanthanum uptake and distribution in internodal cells of Chara[J]. PLANT SCIENCE, PLANT SCIENCE,2008, 2008,174, 174(5):496-501, 496-501. |
| APA | 李子杰.,张智勇.,姜文君.,赵宇亮.,柴之芳.,...&Chai, ZF.(2008).Direct measurement of lanthanum uptake and distribution in internodal cells of Chara.PLANT SCIENCE,174(5),496-501. |
| MLA | 李子杰,et al."Direct measurement of lanthanum uptake and distribution in internodal cells of Chara".PLANT SCIENCE 174.5(2008):496-501. |
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来源：高能物理研究所
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