Chemical speciation analysis of selenium in biological samples by a hyphenated technique of high performance liquid chromatography-inductively coupled plasma mass spectrometry
文献类型：期刊论文
| 作者 | Yu HW(喻宏伟); Chen CY(陈春英); Gao YX(高愈希) ; Li B(李柏); Chai ZF(柴之芳); Yu, HW; Chen, CY; Gao, YX; Li, B; Chai, ZF
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| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
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| 出版日期 | 2006 |
| 卷号 | 34期号:6页码:749-753 |
| 关键词 | reversed ion-pair high performance liquid chromatography inductively coupled plasma mass spectrometry hyphenated technique biological samples speciation analysis selenium |
| 通讯作者 | Chinese Acad Sci, Key Lab Nucl Analyt Tech, Inst High Energy Phys, Beijing 100049, Peoples R China |
| 英文摘要 | A hyphenated technique for chemical speciation analysis of selenium in biological samples was described. A reversed ion-pair high performance liquid chromatograph is introduced for separating selenocompounds, at the meantime an inductively coupled plasma mass spectrometry was combined for on-line elemental determination. A series of standard compounds were used to achieve qualitative and quantitative analysis. Seven seleno-containing compounds, Na2SeO4, Na2SeO3, trimethyl selenonium iodide ( TmSeI ), selenocystaine ( SeCys ), selenourea ( SeUr ), selenomethionine ( SeMet ) and selenocystamine ( SeCM ), can be individually detected. The detection limits are 0.1-1.5 mu g/L, and the linear calibration range for Se concentrations is between 1 and 100 mu g/L. The method was also applied to analyze human liver cytosolic extracts, human serum, and human urine. Only 50 mu L of the samples are needed and the recoveries of spiked seleno-compounds are 93% -117%. The hyphenated method possesses the merits of simple operation, high sensitivity and good repeatability, which is a feasible method for speciation analysis of small molecular selenocompounds in different biological samples. |
| 学科主题 | Chemistry |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 原文出处 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000240869900002 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/238420]  |
| 专题 | 高能物理研究所_院士 高能物理研究所_实验物理中心 高能物理研究所_多学科研究中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Yu HW,Chen CY,Gao YX,et al. Chemical speciation analysis of selenium in biological samples by a hyphenated technique of high performance liquid chromatography-inductively coupled plasma mass spectrometry[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2006,34(6):749-753. |
| APA | 喻宏伟.,陈春英.,高愈希.,李柏.,柴之芳.,...&Chai, ZF.(2006).Chemical speciation analysis of selenium in biological samples by a hyphenated technique of high performance liquid chromatography-inductively coupled plasma mass spectrometry.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,34(6),749-753. |
| MLA | 喻宏伟,et al."Chemical speciation analysis of selenium in biological samples by a hyphenated technique of high performance liquid chromatography-inductively coupled plasma mass spectrometry".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 34.6(2006):749-753. |
入库方式： OAI收割
来源：高能物理研究所
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