Quantification of proteins using lanthanide labeling and HPLC/ICP-MS detection
文献类型：期刊论文
| 作者 | Zheng LN(郑令娜) ; Wang M(王萌); Wang HJ(王华建); Wang B(汪冰); Li B(李柏); Zhao YL(赵宇亮); Chai ZF(柴之芳); Feng WY(丰伟悦); Zheng, LN; Wang, M
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| 刊名 | JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
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| 出版日期 | 2011 |
| 卷号 | 26期号:6页码:1233-1236 |
| 通讯作者 | [Zheng, Ling-Na ; Wang, Meng ; Wang, Hua-Jian ; Wang, Bing ; Li, Bai ; Zhao, Yu-Liang ; Chai, Zhi-Fang ; Feng, Wei-Yue] Chinese Acad Sci, Key Lab Nucl Analyt Tech, Beijing 100049, Peoples R China ; [Zheng, Ling-Na ; Wang, Meng ; Wang, Hua-Jian ; Wang, Bing ; Li, Bai ; Zhao, Yu-Liang ; Chai, Zhi-Fang ; Feng, Wei-Yue] Chinese Acad Sci, Inst High Energy Phys, Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100049, Peoples R China ; [Zheng, Ling-Na ; Li, Jian-Jun] Zhengzhou Univ, Dept Chem, Zhengzhou 450001, Peoples R China |
| 英文摘要 | Quantitative proteomics poses a great challenge and also offers considerable opportunities for analytical chemistry. Currently, the available methods for quantitative proteomics are mainly based on labeling by isotopes and quantification by biological mass spectrometers with soft-ionization sources. Recently, inductively coupled plasma-mass spectrometry (ICP-MS) has been introduced as an attractive complement to biological mass spectrometry for protein quantification. Here we developed a new method based on lanthanide labeling and ICP-MS detection for relative quantification of protein mixtures. The bifunctional reagent DTPAA was chosen as the element-labeling reagent for proteins. Two samples containing RNase A, cytochrome c, and lysozyme in different mixture ratios were labeled with two lanthanides, Ce and Sm, respectively. After separation with cation exchange chromatography, the proteins could be relatively quantified by comparison between signal intensities of Ce and Sm in ICP-MS. The intact proteins can be quantitatively analyzed without enzyme digestion. Because there are 17 lanthanides available for protein labeling, the developed method provides a possibility for high-throughput and top-down proteomics quantification by ICP-MS. |
| 学科主题 | Chemistry; Spectroscopy |
| 类目[WOS] | Chemistry, Analytical ; Spectroscopy |
| 研究领域[WOS] | Chemistry ; Spectroscopy |
| 原文出处 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000290999000015 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/239215]  |
| 专题 | 高能物理研究所_院士 高能物理研究所_实验物理中心 高能物理研究所_加速器中心 高能物理研究所_多学科研究中心 中国科学院高能物理研究所_中国散裂中子源 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Zheng LN,Wang M,Wang HJ,et al. Quantification of proteins using lanthanide labeling and HPLC/ICP-MS detection[J]. JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY,2011,26(6):1233-1236. |
| APA | 郑令娜.,王萌.,王华建.,汪冰.,李柏.,...&Feng, WY.(2011).Quantification of proteins using lanthanide labeling and HPLC/ICP-MS detection.JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY,26(6),1233-1236. |
| MLA | 郑令娜,et al."Quantification of proteins using lanthanide labeling and HPLC/ICP-MS detection".JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY 26.6(2011):1233-1236. |
入库方式： OAI收割
来源：高能物理研究所
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