Atomic force microscopy observation on nuclear reassembly in a cell-free system
文献类型：期刊论文
| 作者 | Yang N(杨宁); Chen ZC(陈忠才); Tang XW(唐孝威) ; Yang, N; Chen, ZC; Zhang, ZH; Zhu, X; Zhai, ZH; Tang, XW
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| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2003 |
| 卷号 | 48期号:21页码:2284-2287 |
| 关键词 | atomic force microscopy nuclear reassembly cell-free system |
| 通讯作者 | Peking Univ, Sch Phys, Beijing 100871, Peoples R China ; Zhejiang Univ, Dept Phys, Hangzhou 310027, Peoples R China ; Peking Univ, Coll Life Sci, Dept Cell Biol & Genet, Beijing 100871, Peoples R China ; Acad Sinica, Inst High Energy Phys, Beijing 100039, Peoples R China |
| 英文摘要 | Cell-free system is interesting and useful for studying nuclear assembly in mitosis. Atomic force microscopy (AFM), which is a simple way for imaging fixed reassemble nuclei with high resolution, has not been used in the cell-free system. In this paper, we put forward an air-drying sample preparation for AFM. Using AFM, we observed nuclear reassembly process within 100 nm resolution in a cell-free system. As a result, we found that the images were artifact-free, and with higher resolution compared with fluorescent optical microscope images. Furthermore, the morphology of membrane vesicles was obtained clearly, and a dynamic change of morphology during the vesicles' approaching to nuclear envelope was also observed, which is enlightened to understand the mechanism of nuclear envelope assembly. |
| 学科主题 | Science & Technology - Other Topics |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 原文出处 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000186533700003 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/239710]  |
| 专题 | 高能物理研究所_院士 高能物理研究所_粒子天体物理中心 中国科学院高能物理研究所_中国散裂中子源 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Yang N,Chen ZC,Tang XW,et al. Atomic force microscopy observation on nuclear reassembly in a cell-free system[J]. CHINESE SCIENCE BULLETIN,2003,48(21):2284-2287. |
| APA | 杨宁.,陈忠才.,唐孝威.,Yang, N.,Chen, ZC.,...&Tang, XW.(2003).Atomic force microscopy observation on nuclear reassembly in a cell-free system.CHINESE SCIENCE BULLETIN,48(21),2284-2287. |
| MLA | 杨宁,et al."Atomic force microscopy observation on nuclear reassembly in a cell-free system".CHINESE SCIENCE BULLETIN 48.21(2003):2284-2287. |
入库方式： OAI收割
来源：高能物理研究所
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