Organization and characteristics of the major histocompatibility complex class II region in the Yangtze finless porpoise (Neophocaena asiaeorientalis asiaeorientalis)
文献类型：期刊论文
| 作者 | Ruan, Rui1,2; Ruan, Jue3; Wan, Xiao-Ling1,2; Zheng, Yang1,2; Chen, Min-Min1; Zheng, Jin-Song1; Wang, Ding1 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2016-03-02 |
| 卷号 | 6 |
| ISSN号 | 2045-2322 |
| 英文摘要 | Little is known about the major histocompatibility complex (MHC) in the genome of Yangtze finless porpoise (Neophocaena asiaeorientalis asiaeorientalis) (YFP) or other cetaceans. In this study, a highquality YFP bacterial artificial chromosome (BAC) library was constructed. We then determined the organization and characterization of YFP MHC class II region by screening the BAC library, followed by sequencing and assembly of positive BAC clones. The YFP MHC class II region consists of two segregated contigs (218,725 bp and 328,435 bp respectively) that include only eight expressed MHC class II genes, three pseudo MHC genes and twelve non-MHC genes. The YFP has fewer MHC class II genes than ruminants, showing locus reduction in DRB, DQA, DQB, and loss of DY. In addition, phylogenic and evolutionary analyses indicated that the DRB, DQA and DQB genes might have undergone birth-and-death evolution, whereas the DQB gene might have evolved under positive selection in cetaceans. These findings provide an essential foundation for future work, such as estimating MHC genetic variation in the YFP or other cetaceans. This work is the first report on the MHC class II region in cetaceans and offers valuable information for understanding the evolution of MHC genome in cetaceans. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | BAC LIBRARY CONSTRUCTION ; BOTTLE-NOSED DOLPHINS ; MHC CLASS-I ; SEQUENCE VARIATION ; GENOMIC DNA ; DELPHINAPTERUS-LEUCAS ; NUCLEOTIDE-SEQUENCES ; TURSIOPS-TRUNCATUS ; MULTIPLE ALIGNMENT ; GENES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000371171900001 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/27729]  |
| 专题 | 水生生物研究所_水环境工程研究中心 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Chinese Acad Agr Sci, Agr Genomes Inst Shenzhen, Guangzhou 518120, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ruan, Rui,Ruan, Jue,Wan, Xiao-Ling,et al. Organization and characteristics of the major histocompatibility complex class II region in the Yangtze finless porpoise (Neophocaena asiaeorientalis asiaeorientalis)[J]. SCIENTIFIC REPORTS,2016,6. |
| APA | Ruan, Rui.,Ruan, Jue.,Wan, Xiao-Ling.,Zheng, Yang.,Chen, Min-Min.,...&Wang, Ding.(2016).Organization and characteristics of the major histocompatibility complex class II region in the Yangtze finless porpoise (Neophocaena asiaeorientalis asiaeorientalis).SCIENTIFIC REPORTS,6. |
| MLA | Ruan, Rui,et al."Organization and characteristics of the major histocompatibility complex class II region in the Yangtze finless porpoise (Neophocaena asiaeorientalis asiaeorientalis)".SCIENTIFIC REPORTS 6(2016). |
入库方式： OAI收割
来源：水生生物研究所
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