Determination of thiophenols with a novel fluorescence labelling reagent: analysis of industrial wastewater samples with SPE extraction coupled with HPLC
文献类型：期刊论文
| 作者 | Sun, Yanan1,2; Lv, Zhengxian3; Sun, Zhiwei3; Wu, Chuanxiang3; Ji, Zhongyin1,2; You, Jinmao1,3 |
| 刊名 | analytical and bioanalytical chemistry
 |
| 出版日期 | 2016-05-01 |
| 卷号 | 408期号:13页码:3527-3536 |
| 关键词 | Thiophenols 9-(2-Iodoethyl) acridone Derivatization HPLC Fluorescence detection |
| 英文摘要 | a simple, sensitive, and selective high-performance liquid chromatography (hplc) method using 9-(2-iodoethyl)acridone (iea) as a novel fluorescence derivatizing agent for the simultaneous determination of six thiophenols has been developed. an efficient pb2+-modified oasis-mcx cartridge was used and could get good recoveries. iea was successfully used to label thiophenols with high sensitivity and excellent selectivity. the effects of different solvents, ph, and surfactants on fluorescence properties of derivatives were investigated. to obtain the best labeling efficiency, derivatizing parameters including ph value, temperature, and concentration of iea, as well as types of catalysts were also evaluated in detail. under the optimal conditions, the separation could be achieved within 12 min with limits of detection (lods) in the range of 0.6-5.8 mu g l-1 and relative standard deviations (rsds) < 3.9 %. this is the first time that iea was applied to the analysis of thiophenols, and the established method has been successfully applied to the trace level detection of thiophenols in industrial wastewater samples. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | performance liquid-chromatography ; amino-acids ; precolumn derivatization ; fluorometric detection ; plasma thiols ; human blood ; cysteine ; homocysteine ; glutathione ; urine |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000374658400016 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/5748]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Northwest Plateau Inst Biol, Key Lab Tibetan Med Res, Xining 810001, Qinghai, Peoples R China 2.Chinese Acad Sci, Grad Univ, 19A Yuquanlu, Beijing 100049, Peoples R China 3.Qufu Normal Univ, Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Yanan,Lv, Zhengxian,Sun, Zhiwei,et al. Determination of thiophenols with a novel fluorescence labelling reagent: analysis of industrial wastewater samples with SPE extraction coupled with HPLC[J]. analytical and bioanalytical chemistry,2016,408(13):3527-3536. |
| APA | Sun, Yanan,Lv, Zhengxian,Sun, Zhiwei,Wu, Chuanxiang,Ji, Zhongyin,&You, Jinmao.(2016).Determination of thiophenols with a novel fluorescence labelling reagent: analysis of industrial wastewater samples with SPE extraction coupled with HPLC.analytical and bioanalytical chemistry,408(13),3527-3536. |
| MLA | Sun, Yanan,et al."Determination of thiophenols with a novel fluorescence labelling reagent: analysis of industrial wastewater samples with SPE extraction coupled with HPLC".analytical and bioanalytical chemistry 408.13(2016):3527-3536. |
入库方式： OAI收割
来源：西北高原生物研究所
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