Convenient and Sensitive HPLC Method for Determination of Nitrosamines in Foodstuffs Based on Pre-column Fluorescence Labeling
文献类型：期刊论文
| 作者 | Zhao, Mei1; Li, Guoliang1,2; Kong, Weiheng1; Lu, Shuaimin1; Xia, Lian1; Chen, Guang1; Zhao, Xianen1; Wu, Yongning2; You, Jinmao1,3 |
| 刊名 | chromatographia
 |
| 出版日期 | 2016-04-01 |
| 卷号 | 79期号:7-8页码:431-439 |
| 关键词 | Nitrosamines HPLC-FLD Fluorescence labeling Response surface methodology Foodstuffs |
| 英文摘要 | the determination of trace nitrosamines (nas) has received more and more attention due to nas' strong carcinogenicity and wide existence in foodstuffs. but the convenient and sensitive method for simultaneously analyzing multiple nas remains poorly investigated. in the present study, a new method was developed for the determination of four nas in foodstuffs based on fluorescence labeling followed by high-performance liquid chromatography with fluorescence detection (hplc-fld), which did not require complex pretreatments and expensive equipment. nas were firstly denitrosated using hydrobromic acid-acetic acid to obtain the secondary amines. subsequently, a fluorescence labelling procedure was immediately carried out to label the secondary amines using 1,2-benzo-3,4-dihydrocarbazole-9-ethyl chloroformate (bceoc). a response surface methodology (rsm) based on a box-behnken design (bbd) was used to optimize the main parameters affecting the denitrosation. under the optimal hplc conditions, the quantitative linear range of analytes was 0.05-200 nmol l-1, and the correlation coefficients were more than 0.9998. detection limits for all na derivatives were achieved within the range of 1.3-2.5 ng l-1. the proposed method was successfully applied for the determination of trace nas in several foodstuffs. this method was proven to be simple, inexpensive, sensitive, accurate and reliable for trace na determination in foodstuffs. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | performance liquid-chromatography ; volatile n-nitrosamines ; meat-products ; ms/ms method ; gc-ms ; derivatization ; microextraction ; quantification ; identification ; nitrite |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000376124400007 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/5756]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu 273165, Peoples R China 2.China Natl Ctr Food Safety Risk Assessment, Key Labs Chem Safety & Hlth, Beijing 100050, Peoples R China 3.Chinese Acad Sci, Northwest Plateau Inst Biol, Xining 810001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Mei,Li, Guoliang,Kong, Weiheng,et al. Convenient and Sensitive HPLC Method for Determination of Nitrosamines in Foodstuffs Based on Pre-column Fluorescence Labeling[J]. chromatographia,2016,79(7-8):431-439. |
| APA | Zhao, Mei.,Li, Guoliang.,Kong, Weiheng.,Lu, Shuaimin.,Xia, Lian.,...&You, Jinmao.(2016).Convenient and Sensitive HPLC Method for Determination of Nitrosamines in Foodstuffs Based on Pre-column Fluorescence Labeling.chromatographia,79(7-8),431-439. |
| MLA | Zhao, Mei,et al."Convenient and Sensitive HPLC Method for Determination of Nitrosamines in Foodstuffs Based on Pre-column Fluorescence Labeling".chromatographia 79.7-8(2016):431-439. |
入库方式： OAI收割
来源：西北高原生物研究所
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