The requirement for protein kinase C delta (PRKCD) during preimplantation bovine embryo development
文献类型：期刊论文
| 作者 | Yang, Qi-En1,2; Ozawa, Manabu2,4; Zhang, Kun2,5; Johnson, Sally E.2,3; Ealy, Alan D.2,3 |
| 刊名 | reproduction fertility and development
 |
| 出版日期 | 2016 |
| 卷号 | 28期号:4页码:482-490 |
| 关键词 | embryonic development embryonic gene expression post-hatching development |
| 英文摘要 | protein kinase c (pkc) delta (prkcd) is a member of the novel pkc subfamily that regulates gene expression in bovine trophoblast cells. additional functions for prkcd in early embryonic development in cattle have not been fully explored. the objectives of this study were to describe the expression profile of prkcd mrna in bovine embryos and to examine its biological roles during bovine embryo development. both prkcd mrna and protein are present throughout early embryo development and increases in mrna abundance are evident at morula and blastocyst stages. phosphorylation patterns are consistent with detection of enzymatically active prkcd in bovine embryos. exposure to a pharmacological inhibitor (rottlerin) during early embryonic development prevented development beyond the eight- to 16-cell stage. treatment at or after the 16-cell stage reduced blastocyst development rates, total blastomere numbers and inner cell mass-to-trophoblast cell ratio. exposure to the inhibitor also decreased basal interferon tau (ifnt) transcript abundance and abolished fibroblast growth factor-2 induction of ifnt expression. furthermore, trophoblast adhesion and proliferation was compromised in hatched blastocysts. these observations provide novel insights into prkcd mrna expression profiles in bovine embryos and provide evidence for prkcd-dependent regulation of embryonic development, gene expression and post-hatching events. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | developmental biology ; reproductive biology ; zoology |
| 研究领域[WOS] | developmental biology ; reproductive biology ; zoology |
| 关键词[WOS] | interferon-tau expression ; fibroblast growth-factors ; in-vitro ; conceptus development ; blastocyst formation ; mouse embryos ; pkc isoforms ; compaction ; stage ; trophectoderm |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000370856400010 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/5777]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Northwest Plateau Inst Biol, Key Lab Adapt & Evolut Plateau Biota, Xining 810001, Qinghai, Peoples R China 2.Univ Florida, Dept Anim Sci, Gainesville, FL 32611 USA 3.Virginia Polytech Inst & State Univ, Dept Anim & Poultry Sci, 175 W Campus Dr, Blacksburg, VA 24061 USA 4.Univ Tokyo, Inst Med Sci, Tokyo 1088639, Japan 5.Michigan State Univ, Dept Anim Sci, E Lansing, MI 48824 USA |
| 推荐引用方式 GB/T 7714 | Yang, Qi-En,Ozawa, Manabu,Zhang, Kun,et al. The requirement for protein kinase C delta (PRKCD) during preimplantation bovine embryo development[J]. reproduction fertility and development,2016,28(4):482-490. |
| APA | Yang, Qi-En,Ozawa, Manabu,Zhang, Kun,Johnson, Sally E.,&Ealy, Alan D..(2016).The requirement for protein kinase C delta (PRKCD) during preimplantation bovine embryo development.reproduction fertility and development,28(4),482-490. |
| MLA | Yang, Qi-En,et al."The requirement for protein kinase C delta (PRKCD) during preimplantation bovine embryo development".reproduction fertility and development 28.4(2016):482-490. |
入库方式： OAI收割
来源：西北高原生物研究所
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