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A fluorescence resonance energy transfer (FRET) based "Turn-On" nanofluorescence sensor using a nitrogen-doped carbon dot-hexagonal cobalt oxyhydroxide nanosheet architecture and application to alpha-glucosidase inhibitor screening
文献类型：期刊论文
| 作者 | Li, Guoliang1,2; Kong, Weiheng1; Zhao, Mei1; Lu, Shuaimin1; Gong, Peiwei1; Chen, Guang1; Xia, Lian1; Wang, Hua1; You, Jinmao1,3; Wu, Yongning2 |
| 刊名 | biosensors & bioelectronics
 |
| 出版日期 | 2016-05-15 |
| 卷号 | 79页码:728-735 |
| 关键词 | Nitrogen-doped carbon dots alpha-Glucosidase Enzyme inhibition Fluorescence resonance energy transfer |
| 英文摘要 | the medicines targeted at alpha-glucosidase played an important role in anti-diabetes and anti-hiv therapy. unfortunately, the method based on fluorescent assay strategy for alpha-glucosidase inhibitor screening remains poorly investigated. in this study, a novel "turn on" fluorescence sensor platform has been developed for trace alpha-glucosidase inhibitor screening from natural medicines. firstly, carbon dots were prepared by one-pot synthesis and used as the signal output. combining with the carbon dots, cobalt oxyhydroxide (coooh) nanoflakes were employed to build the fluorescence resonance energy transfer (fret) based sensor platform. secondly, l-ascorbic acid-2-o-alpha-d-glucopyranosyl (aag) was innovatively introduced as alpha-glucosidase substrate. with hydrolysis of aag by alpha-glucosidase, ascorbic acids (aa) were released that can rapidly reduce coooh nanoflakes to co2+, and then fret was stopped accompanying with the fluorescence recovery of cds. the sensor platform was ultrasensitive to aa with a detection limit of 5 nm, ensuring the sensitive monitoring of enzyme activity. acarbose was used as the inhibitor model and its inhibition rate is proportional to the logarithm of concentration in range of 10(-9)-10(-3) m with the correlation coefficient of r-2=0.996, and an ultralow limit of detection of 1 x 10(-9) m was obtained. the inhibiting ability of seven compounds isolated from natural medicines was also evaluated. the constructed sensor platform was proven to be sensitive and selective as well as cost-effective, facile and reliable, making it promising as a candidate for trace alpha-glucosidase inhibitor screening. (c) 2015 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biophysics ; biotechnology & applied microbiology ; chemistry, analytical ; electrochemistry ; nanoscience & nanotechnology |
| 研究领域[WOS] | biophysics ; biotechnology & applied microbiology ; chemistry ; electrochemistry ; science & technology - other topics |
| 关键词[WOS] | quantum dots ; ascorbic-acid ; drinking-water ; probe ; nanoparticles ; luminescence ; green |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000370309200100 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/5779]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu 273165, Peoples R China 2.China Natl Ctr Food Safety Risk Assessment, Key Labs Chem Safety & Hlth, Beijing 100050, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Guoliang,Kong, Weiheng,Zhao, Mei,et al. A fluorescence resonance energy transfer (FRET) based "Turn-On" nanofluorescence sensor using a nitrogen-doped carbon dot-hexagonal cobalt oxyhydroxide nanosheet architecture and application to alpha-glucosidase inhibitor screening[J]. biosensors & bioelectronics,2016,79:728-735. |
| APA | Li, Guoliang.,Kong, Weiheng.,Zhao, Mei.,Lu, Shuaimin.,Gong, Peiwei.,...&Wu, Yongning.(2016).A fluorescence resonance energy transfer (FRET) based "Turn-On" nanofluorescence sensor using a nitrogen-doped carbon dot-hexagonal cobalt oxyhydroxide nanosheet architecture and application to alpha-glucosidase inhibitor screening.biosensors & bioelectronics,79,728-735. |
| MLA | Li, Guoliang,et al."A fluorescence resonance energy transfer (FRET) based "Turn-On" nanofluorescence sensor using a nitrogen-doped carbon dot-hexagonal cobalt oxyhydroxide nanosheet architecture and application to alpha-glucosidase inhibitor screening".biosensors & bioelectronics 79(2016):728-735. |
入库方式： OAI收割
来源：西北高原生物研究所
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