Physiological and transcriptional responses of two contrasting Populus clones to nitrogen stress
文献类型：期刊论文
| 作者 | Wang, Xiaoli1,2; Li, Xiaodong2; Zhang, Sheng3 ; Korpelainen, Helena4; Li, Chunyang5
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| 刊名 | TREE PHYSIOLOGY
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| 出版日期 | 2016-05-01 |
| 卷号 | 36期号:5页码:628-642 |
| 关键词 | gene expression profiling miRNAs |
| ISSN号 | 0829-318X |
| 通讯作者 | Li, Chunyang |
| 英文摘要 | The aim of this study was to reveal mechanisms responsible for nitrogen (N) stress in two contrasting Populus clones. Leaves of Nanlin 1388 (N stress-insensitive clone hybrids of Populus deltoides Bart.CV.aEuro...xaEuro...Populus euramericana (Dode) Guineir CV) and Nanlin 895 (N stress-sensitive clone hybrids of Populus deltoides Bart.CV.aEuro...xaEuro...Populus euramericana (Dode) Guineir CV) were harvested and analyzed. Different responses visible in photosynthesis, N and carbon contents, physiological traits, and chlorophyll were observed. The Solexa/Illumina's digital gene expression system was used to investigate differentially expressed miRNAs and mRNAs under N stress. Target profiling, and biological network and function analyses were also performed. Randomly selected mRNAs and miRNAs were validated by quantitative reverse transcription polymerase chain reaction. In all, 110 Nanlin 1388 and 122 Nanlin 895 miRNAs were differentially expressed, among which 34 and 23 miRNAs were newly found in the two clones, respectively. Under N stress, a total of 329 and 98 mRNAs were regulated in N stress-insensitive and -sensitive clones, respectively. Notably, the miR396 family and its regulated mRNAs were altered in both clones under N stress, while miR646 was regulated only in the N stress-insensitive clone (Nanlin 1388), and miR156, miR319 and miR393 in the N stress-sensitive clone (Nanlin 895). Gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses also proved several clone-specific functions and pathways. These findings may be significant for understanding the genetic responses of Populus to N stress. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Forestry |
| 研究领域[WOS] | Forestry |
| 关键词[WOS] | SMALL RNAS ; OXIDATIVE STRESS ; ARABIDOPSIS ; MICRORNAS ; PLANTS ; NUTRIENT ; IDENTIFICATION ; STARVATION ; GROWTH ; GENE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000377403300009 |
| 源URL | [http://ir.imde.ac.cn/handle/131551/17378]  |
| 专题 | 成都山地灾害与环境研究所_山地表生过程与生态调控重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Chengdu Inst Biol, Chengdu 610041, Peoples R China 2.Guizhou Acad Agr Sci, Guizhou Inst Prataculture, Guiyang 550006, Guizhou, Peoples R China 3.Chinese Acad Sci, Inst Mt Hazards & Environm, Chengdu 610041, Peoples R China 4.Univ Helsinki, Viikki Plant Sci Ctr, Dept Agr Sci, POB 27, FI-00014 Helsinki, Finland 5.Zhejiang A&F Univ, Nurturing Stn, State Key Lab Subtrop Silviculture, Linan 311300, Zhejiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xiaoli,Li, Xiaodong,Zhang, Sheng,et al. Physiological and transcriptional responses of two contrasting Populus clones to nitrogen stress[J]. TREE PHYSIOLOGY,2016,36(5):628-642. |
| APA | Wang, Xiaoli,Li, Xiaodong,Zhang, Sheng,Korpelainen, Helena,&Li, Chunyang.(2016).Physiological and transcriptional responses of two contrasting Populus clones to nitrogen stress.TREE PHYSIOLOGY,36(5),628-642. |
| MLA | Wang, Xiaoli,et al."Physiological and transcriptional responses of two contrasting Populus clones to nitrogen stress".TREE PHYSIOLOGY 36.5(2016):628-642. |
入库方式： OAI收割
来源：成都山地灾害与环境研究所
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