Cross-Interface Emulsification for Generating Size-Tunable Droplets
文献类型：期刊论文
| 作者 | Xu P; Zheng X(郑旭); Tao Y ; Du WB
|
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2016 |
| 卷号 | 88期号:6页码:3171-3177 |
| 通讯作者邮箱 | wenbin@im.ac.cn |
| ISSN号 | 0003-2700 |
| 产权排序 | [Xu, Peng; Tao, Yi; Du, Wenbin] Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China; [Zheng, Xu] Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing 100190, Peoples R China |
| 通讯作者 | Du, WB (reprint author), Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China. |
| 中文摘要 | We report cross-interface emulsification (XiE), a pimple method for the generation of monodisperse droplets of controllable volumes from picoliter to nanoliter. A device is set up in which a fused-silica capillary Is vibrating across the surface of the continuous phase (mineral oil) in a reservoir, and the flow of the dispersed phase (aqueous solution) in the capillary is segmented into monodisperse droplets at the air/oil interface. We, find that the volume of droplets is mainly dominated by the flow rate and vibrating frequency and not significantly influenced by other factors,, such as the viscosity of the continuous phase and dispersed phase, the inner diameter of the capillary (20-100 mu m), Or the shape of the tip (tapered or flat). These features reflect high robustness, flexibility, and precision of XiE, for on-demand volume control of droplets. The droplets automatically assemble into planar monolayer droplet arrays (PMDA) in flat-bottomed microwells of 96-well plates, offering excellent convenience for imaging of droplets. As a representative application, we carry out digital loop-mediated isothermal amplification using PMDAs with multivolume droplets for the absolute quantification of nucleic acids. Our results demonstrate that XiE is simple and controllable for the production of monodisperse size-tunable droplets, and it offers opportunities for common laboratories, even without microfabrication facilities, to perform digital quantification, single cell analysis, and other biochemical assays with high throughput. |
| 分类号 | 一类 |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 收录类别 | SCI |
| 原文出处 | http://dx.doi.org/10.1021/acs.analchem.5b04510 |
| 语种 | 英语 |
| WOS记录号 | WOS:000372391500028 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/59519]  |
| 专题 | 力学研究所_非线性力学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Xu P,Zheng X,Tao Y,et al. Cross-Interface Emulsification for Generating Size-Tunable Droplets[J]. ANALYTICAL CHEMISTRY,2016,88(6):3171-3177. |
| APA | Xu P,郑旭,Tao Y,&Du WB.(2016).Cross-Interface Emulsification for Generating Size-Tunable Droplets.ANALYTICAL CHEMISTRY,88(6),3171-3177. |
| MLA | Xu P,et al."Cross-Interface Emulsification for Generating Size-Tunable Droplets".ANALYTICAL CHEMISTRY 88.6(2016):3171-3177. |
入库方式： OAI收割
来源：力学研究所
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