Label-free multiplex immunoassay of AFP, CEA and CA19-9 by integrated microfluidic biosensor based on imaging ellipsometry
文献类型：期刊论文
| 作者 | Huang CH; Yang QL; Song FY; Chen N ; Yao B; Liao XL; Zhang ST; Chen YY; Jin G(靳刚)
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| 刊名 | INTEGRATED FERROELECTRICS
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| 出版日期 | 2016 |
| 卷号 | 171期号:1页码:59-69 |
| 通讯作者邮箱 | chhuang2007@sinano.ac.cn ; yychen2006@sinano.ac.cn ; gajin@imech.ac.cn |
| 关键词 | Imaging ellipsometry immunoassay AFP CEA CA19-9 |
| ISSN号 | 1058-4587 |
| 产权排序 | [Huang, Chenghong; Yang, QingLin; Chen, Nan; Yao, Bo; Liao, Xiaoling; Zhang, ShiTong] Chongqing Univ Sci & Technol, Inst Biomat & Living Cell Imaging Technol, Chongqing, Peoples R China; [Song, Fayi] Univ Toronto, Inst Biomat & Biomed Engn, Toronto, ON, Canada; [Chen, Yanyan] Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou, Peoples R China; [Jin, Gang] Chinese Acad Sci, Inst Mech, Beijing, Peoples R China |
| 通讯作者 | Huang, CH (reprint author), Chongqing Univ Sci & Technol, Inst Biomat & Living Cell Imaging Technol, Chongqing, Peoples R China. ; Chen, YY (reprint author), Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou, Peoples R China. ; Jin, G (reprint author), Chinese Acad Sci, Inst Mech, Beijing, Peoples R China. |
| 中文摘要 | This study describes the development of alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA) and carbohydrate 19-9(CA19-9) multiplex immunoassay by integrated microfluidic device based on imaging ellipsometry (IMBIE). Different strategies for AFP, CEA and CA 19-9 simultaneous immunoassay on the same aldehyde-substrate were developed. Unifying the reaction steps of CA19-9 for antigen direct detection, CEA for secondary antibody (rabbit-antiCEA) addition, AFP for secondary (rabbit antiAFP) and an enhancer (goat IgG) application, and comprehensively considering the dynamic ranges and detection sensitivities, we combined these three markers into multiplex analysis in serial format.The sensitivities and dynamic ranges for CA19-9, CEA and AFP were 10.0U/mL, 1.0 ng/mL, 1.0 ng/mL and 2.0-100.0 U/mL, 0.1-64.0 ng/mL, 1.0-128.0 ng/mL, respectively. The intra-assay and inter-assay (coefficient variations) CVs of CA19-9, CEA and AFP were between 1.6-8.9% and 2.1-7.8%. The specificities tested for both cross-reaction and heterogeneous proteins of CA19-9, CEA and AFP were not more than 6.7%. Quantitative detection of 49 sera from gastric patients demonstrated that the accuracy for gastric cancer diagnosis was 83.4%. The total detection time is only 60min for an automated process. The proposed assay has the potential for practical application. |
| 分类号 | Q4 |
| 类目[WOS] | Engineering, Electrical & Electronic ; Physics, Applied ; Physics, Condensed Matter |
| 研究领域[WOS] | Engineering ; Physics |
| 关键词[WOS] | Imaging ellipsometry ; immunoassay ; AFP ; CEA ; CA19-9 |
| 收录类别 | SCI ; EI |
| 原文出处 | http://dx.doi.org/10.1080/10584587.2016.1171664 |
| 语种 | 英语 |
| WOS记录号 | WOS:000378406600009 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/59532]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Huang CH,Yang QL,Song FY,et al. Label-free multiplex immunoassay of AFP, CEA and CA19-9 by integrated microfluidic biosensor based on imaging ellipsometry[J]. INTEGRATED FERROELECTRICS,2016,171(1):59-69. |
| APA | Huang CH.,Yang QL.,Song FY.,Chen N.,Yao B.,...&靳刚.(2016).Label-free multiplex immunoassay of AFP, CEA and CA19-9 by integrated microfluidic biosensor based on imaging ellipsometry.INTEGRATED FERROELECTRICS,171(1),59-69. |
| MLA | Huang CH,et al."Label-free multiplex immunoassay of AFP, CEA and CA19-9 by integrated microfluidic biosensor based on imaging ellipsometry".INTEGRATED FERROELECTRICS 171.1(2016):59-69. |
入库方式： OAI收割
来源：力学研究所
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