Cholesterol-based cationic lipids for gene delivery: Contribution of molecular structure factors to physico-chemical and biological properties
文献类型：期刊论文
| 作者 | Sheng RL(盛瑞隆); Luo T(罗挺); Li H(李慧); Sun JJ(孙景景); Wang Z(王昭); Cao AM(曹阿民) |
| 刊名 | Colloid Surf. B-Biointerfaces
 |
| 出版日期 | 2014 |
| 卷号 | 116页码:32-40 |
| 其他题名 | 基于甾体化合物的脂质体基因载体: 分子结构因子对物理化学性能和生物性能的贡献 |
| 通讯作者 | 曹阿民 |
| 英文摘要 | In this work, we prepared a series of cholesterol-based cationic (Cho-cat) lipids bearing cholesterol hydrophobe, natural amino acid headgroups (lysine/histidine) and linkage (carbonate ester/ether) bonds. In which, the natural amino acid headgroups made dominant contribution to their physico-chemical and biological properties. Among the lipids, the L-lysine headgroup bearing lipids (Cho-es/et-Lys) showed higher pDNA binding affinity and were able to form larger sized and higher surface charged lipoplexes than that of L-histidine headgroup bearing lipids (Cho-es/et-His), they also demonstrated higher transfection efficacy and higher membrane disruption capacities than that of their L-histidine headgroup bearing counterparts. However, compared to the contributions of the headgroups, the (carbonate ester/ether) linkage bonds showed much less affects. Besides, it could be noted that, Cho-es/et-Lys lipids exhibited very high luciferase gene transfection efficiency that almost reached the transfection level of "gold standard" bPEI-25k, made them potential transfection reagents for practical application. Moreover, the results facilitated the understanding for the structure activity relationship of the cholesterol-based cationic lipids, and also paved a simple and efficient way for achieving high transfection efficiency by modification of suitable headgroups on lipid gene carriers. (C) 2014 Elsevier B.V. All rights reserved. |
| 学科主题 | 高分子化学 |
| 收录类别 | SCI |
| 原文出处 | http://dx.doi.org/10.1016/j.colsurfb.2013.12.039 |
| 语种 | 英语 |
| 源URL | [http://ir.sioc.ac.cn/handle/331003/38892]  |
| 专题 | 上海有机化学研究所_高分子材料研究室 |
| 作者单位 | 中科院上海有机化学研究所 |
| 推荐引用方式 GB/T 7714 | Sheng RL,Luo T,Li H,et al. Cholesterol-based cationic lipids for gene delivery: Contribution of molecular structure factors to physico-chemical and biological properties[J]. Colloid Surf. B-Biointerfaces,2014,116:32-40. |
| APA | 盛瑞隆,罗挺,李慧,孙景景,王昭,&曹阿民.(2014).Cholesterol-based cationic lipids for gene delivery: Contribution of molecular structure factors to physico-chemical and biological properties.Colloid Surf. B-Biointerfaces,116,32-40. |
| MLA | 盛瑞隆,et al."Cholesterol-based cationic lipids for gene delivery: Contribution of molecular structure factors to physico-chemical and biological properties".Colloid Surf. B-Biointerfaces 116(2014):32-40. |
入库方式： OAI收割
来源：上海有机化学研究所
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