Characterization of AvaR1, an autoregulator receptor that negatively controls avermectins production in a high avermectin-producing strain
文献类型：期刊论文
| 作者 | Wang JB(王健博); Zhang F(张凤); Pu JY(蒲津越); Zhao J(赵娟); Zhao QF(赵群飞); Tang GL(唐功利) |
| 刊名 | Biotechnol. Lett.
 |
| 出版日期 | 2014 |
| 卷号 | 36期号:4页码:813-819 |
| 其他题名 | 阿维菌素高产菌中AvaR作为一个自调控受体负调控阿维菌素产生 |
| 通讯作者 | 唐功利 |
| 英文摘要 | Many gamma-butyrolactone-autoregulator receptors control the production of secondary metabolites in Streptomyces spp. Hence, AvaR1, an autoregulator receptor protein in Streptomyces avermitilis, was characterized as a negative regulator of avermectin (Ave) production. Deletion of AvaR1 in a high-producing strain increased production of Ave B1a approx. 1.75 times (similar to 700 mu g/ml) compared with the parent strain. Semi-quantitative RT-PCR and electrophoretic mobility shift assays revealed that AvaR1 regulates the biosynthesis of Ave but not through the aveR pathway-specific regulatory gene. A special signaling molecule, avenolide, increased production of Ave. This study has refined our understanding of how avenolide regulates the production of Aves which is promising for developing new methods to improve the production of antibiotics in industrial strains. |
| 学科主题 | 生命有机化学 |
| 收录类别 | SCI |
| 原文出处 | http://dx.doi.org/10.1007/s10529-013-1416-y |
| 语种 | 英语 |
| 源URL | [http://ir.sioc.ac.cn/handle/331003/38936]  |
| 专题 | 上海有机化学研究所_生命有机化学国家重点实验室 |
| 作者单位 | 中科院上海有机化学研究所 |
| 推荐引用方式 GB/T 7714 | Wang JB,Zhang F,Pu JY,et al. Characterization of AvaR1, an autoregulator receptor that negatively controls avermectins production in a high avermectin-producing strain[J]. Biotechnol. Lett.,2014,36(4):813-819. |
| APA | 王健博,张凤,蒲津越,赵娟,赵群飞,&唐功利.(2014).Characterization of AvaR1, an autoregulator receptor that negatively controls avermectins production in a high avermectin-producing strain.Biotechnol. Lett.,36(4),813-819. |
| MLA | 王健博,et al."Characterization of AvaR1, an autoregulator receptor that negatively controls avermectins production in a high avermectin-producing strain".Biotechnol. Lett. 36.4(2014):813-819. |
入库方式： OAI收割
来源：上海有机化学研究所
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