Dietary alpha-linolenic acid/linoleic acid ratios modulate intestinal immunity, tight junctions, anti-oxidant status and mRNA levels of NF-kappa B p65, MLCK and Nrf2 in juvenile grass carp (Ctenopharyngodon idella)
文献类型：期刊论文
| 作者 | Zeng, Yun-Yun1; Jiang, Wei-Dan1,2,3; Liu, Yang1,2,3; Wu, Pei1,2,3; Zhao, Juan1; Jiang, Jun1,2,3; Kuang, Sheng-Yao4; Tang, Ling4; Tang, Wu-Neng4; Zhang, Yong-An5 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2016-04-01 |
| 卷号 | 51期号:*页码:351-364 |
| 关键词 | Alpha-linolenic acid Linoleic acid Grass carp Immune response Tight junction proteins Antioxidant status Intestine |
| ISSN号 | 1050-4648 |
| 英文摘要 | This study was conducted to investigate the effects of dietary alpha-linolenic acid/linoleic acid (ALA/LNA) ratios on the immune response, tight junctions, antioxidant status and immune-related signaling molecules mRNA levels in the intestine of juvenile grass carp (Ctenopharyngodon idella). A total of 1260 juvenile grass carp with an average initial weight of 8.78 +/- 0.03 g were fed diets with different ALA/LNA ratios (0.01, 0.34, 0.68, 1.03, 1.41, 1.76 and 2.15) for 60 days. Results indicated that ALA/LNA ratio of 1.03 significantly increased acid phosphatase, lysozyme activities and complement C3 contents, promoted interleukin 10, transforming growth factor beta 1 and KB inhibitor alpha mRNA abundance, whereas suppressed pro-inflammatory cytokines (interleukin 1 beta, interleukin 8, tumor necrosis factor alpha and interferon gamma 2) and signal molecules (I kappa B kinase beta, I kappa B kinase gamma and nuclear factor kappa B p65) mRNA levels in the intestine (P < 0.05), suggesting that optimal dietary ALA/LNA ratio improved intestinal immune response of juvenile fish. Additionally, ALA/LNA ratio of 1.03 significantly promoted Claudin-3, Claudin-b, Claudin-c, Occludin and ZO-1 gene transcription, whereas reduced Claudin-15a and myosin light-chain kinase mRNA levels in the intestine, suggesting that appropriate dietary ALA/LNA ratio strengthened tight junctions in the intestine of juvenile fish. Meanwhile, ALA/LNA ratio of 1.03 noticeably elevated glutathione contents, copper/zinc superoxide dismutase, glutathione peroxidase, glutathione S-transferase and glutathione reductase activities and mRNA levels, as well as signaling molecule nuclear factor erythoid 2-related factor 2 gene transcriptional abundance in the intestine, suggesting that proper, yatio of dietary ALA/LNA ameliorate the intestinal antioxidant status of juvenile fish. Based on the quadratic regression analysis of the complement C3 content in the distal intestine and malondialdehyde content in the whole intestine, optimal ALA/LNA ratio for maximum growth of juvenile grass carp (8.78-72.00 g) were estimated to be 1.13 and 1.12, respectively. (C) 2015 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 关键词[WOS] | POLYUNSATURATED FATTY-ACIDS ; GROWTH-PERFORMANCE ; OXIDATIVE STRESS ; EPITHELIAL-CELLS ; MANNAN OLIGOSACCHARIDES ; EXPRESSION ANALYSIS ; ENZYME-ACTIVITIES ; LIPID-METABOLISM ; ATLANTIC SALMON ; PROTEIN GENES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000374617700039 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/27968]  |
| 专题 | 水生生物研究所_水生生物多样性与资源保护研究中心 |
| 作者单位 | 1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Peoples R China 2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Chengdu 611130, Peoples R China 3.Sichuan Agr Univ, Key Lab Anim Dis Resistance Nutr, China Minist Educ, Chengdu 611130, Peoples R China 4.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 610066, Peoples R China 5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zeng, Yun-Yun,Jiang, Wei-Dan,Liu, Yang,et al. Dietary alpha-linolenic acid/linoleic acid ratios modulate intestinal immunity, tight junctions, anti-oxidant status and mRNA levels of NF-kappa B p65, MLCK and Nrf2 in juvenile grass carp (Ctenopharyngodon idella)[J]. FISH & SHELLFISH IMMUNOLOGY,2016,51(*):351-364. |
| APA | Zeng, Yun-Yun.,Jiang, Wei-Dan.,Liu, Yang.,Wu, Pei.,Zhao, Juan.,...&Feng, Lin.(2016).Dietary alpha-linolenic acid/linoleic acid ratios modulate intestinal immunity, tight junctions, anti-oxidant status and mRNA levels of NF-kappa B p65, MLCK and Nrf2 in juvenile grass carp (Ctenopharyngodon idella).FISH & SHELLFISH IMMUNOLOGY,51(*),351-364. |
| MLA | Zeng, Yun-Yun,et al."Dietary alpha-linolenic acid/linoleic acid ratios modulate intestinal immunity, tight junctions, anti-oxidant status and mRNA levels of NF-kappa B p65, MLCK and Nrf2 in juvenile grass carp (Ctenopharyngodon idella)".FISH & SHELLFISH IMMUNOLOGY 51.*(2016):351-364. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。