Novel Two-Step Hierarchical Screening of Mutant Pools Reveals Mutants under Selection in Chicks
文献类型：期刊论文
| 作者 | Yang, Hee-Jeong1; Bogomolnaya, Lydia M.1,4; Elfenbein, Johanna R.1,5; Endicott-Yazdani, Tiana1; Reynolds, M. Megan2; Porwollik, Steffen3; Cheng, Pui3; Xia, Xiao-Qin3,6; McClelland, Michael3; Andrews-Polymenis, Helene1 |
| 刊名 | INFECTION AND IMMUNITY
 |
| 出版日期 | 2016-04-01 |
| 卷号 | 84期号:4页码:1226-1238 |
| ISSN号 | 0019-9567 |
| 英文摘要 | Contaminated chicken/egg products are major sources of human salmonellosis, yet the strategies used by Salmonella to colonize chickens are poorly understood. We applied a novel two-step hierarchical procedure to identify new genes important for colonization and persistence of Salmonella enterica serotype Typhimurium in chickens. A library of 182 S. Typhimurium mutants each containing a targeted deletion of a group of contiguous genes (for a total of 2,069 genes deleted) was used to identify regions under selection at 1, 3, and 9 days postinfection in chicks. Mutants in 11 regions were under selection at all assayed times (colonization mutants), and mutants in 15 regions were under selection only at day 9 (persistence mutants). We assembled a pool of 92 mutants, each deleted for a single gene, representing nearly all genes in nine regions under selection. Twelve single gene deletion mutants were under selection in this assay, and we confirmed 6 of 9 of these candidate mutants via competitive infections and complementation analysis in chicks. STM0580, STM1295, STM1297, STM3612, STM3615, and STM3734 are needed for Salmonella to colonize and persist in chicks and were not previously associated with this ability. One of these key genes, STM1297 (selD), is required for anaerobic growth and supports the ability to utilize formate under these conditions, suggesting that metabolism of formate is important during infection. We report a hierarchical screening strategy to interrogate large portions of the genome during infection of animals using pools of mutants of low complexity. Using this strategy, we identified six genes not previously known to be needed during infection in chicks, and one of these (STM1297) suggests an important role for formate metabolism during infection. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Immunology ; Infectious Diseases |
| 研究领域[WOS] | Immunology ; Infectious Diseases |
| 关键词[WOS] | ENTERICA SEROVAR TYPHIMURIUM ; SIGNAL PEPTIDE PEPTIDASE ; MICROARRAY DATA-ANALYSIS ; SELD GENE-PRODUCT ; SALMONELLA-ENTERICA ; ESCHERICHIA-COLI ; SEROTYPE TYPHIMURIUM ; FORMATE DEHYDROGENASE ; UNITED-STATES ; INTESTINAL COLONIZATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000377103600034 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/27983]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心 |
| 作者单位 | 1.Texas A&M Univ Syst, Coll Med, Hlth Sci Ctr, Dept Microbial Pathogenesis & Immunol, Bryan, TX USA 2.Texas A&M Univ, Dept Biochem & Biophys, Coll Agr & Life Sci, College Stn, TX 77843 USA 3.Univ Calif Irvine, Dept Microbiol & Mol Genet, Irvine, CA 92717 USA 4.Kazan Fed Univ, Inst Fundamental Med & Biol, Kazan, Russia 5.N Carolina State Univ, Coll Vet Med, Dept Clin Sci, Raleigh, NC USA 6.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Hee-Jeong,Bogomolnaya, Lydia M.,Elfenbein, Johanna R.,et al. Novel Two-Step Hierarchical Screening of Mutant Pools Reveals Mutants under Selection in Chicks[J]. INFECTION AND IMMUNITY,2016,84(4):1226-1238. |
| APA | Yang, Hee-Jeong.,Bogomolnaya, Lydia M..,Elfenbein, Johanna R..,Endicott-Yazdani, Tiana.,Reynolds, M. Megan.,...&Andrews-Polymenis, Helene.(2016).Novel Two-Step Hierarchical Screening of Mutant Pools Reveals Mutants under Selection in Chicks.INFECTION AND IMMUNITY,84(4),1226-1238. |
| MLA | Yang, Hee-Jeong,et al."Novel Two-Step Hierarchical Screening of Mutant Pools Reveals Mutants under Selection in Chicks".INFECTION AND IMMUNITY 84.4(2016):1226-1238. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。