Characterization of type 2 diacylglycerol acyltransferases in Chlamydomonas reinhardtii reveals their distinct substrate specificities and functions in triacylglycerol biosynthesis
文献类型：期刊论文
| 作者 | Liu, Jin1,2,3; Han, Danxiang4; Yoon, Kangsup4; Hu, Qiang4; Li, Yantao1,2 |
| 刊名 | PLANT JOURNAL
 |
| 出版日期 | 2016-04-01 |
| 卷号 | 86期号:1页码:3-19 |
| 关键词 | Chlamydomonas reinhardtii diacylglycerol acyltransferase lipid metabolism triacylglycerol algae biofuels |
| ISSN号 | 0960-7412 |
| 英文摘要 | Diacylglycerol acyltransferases (DGATs) catalyze a rate-limiting step of triacylglycerol (TAG) biosynthesis in higher plants and yeast. The genome of the green alga Chlamydomonas reinhardtii has multiple genes encoding type 2 DGATs (DGTTs). Here we present detailed functional and biochemical analyses of Chlamydomonas DGTTs. In vitro enzyme analysis using a radiolabel-free assay revealed distinct substrate specificities of three DGTTs: CrDGTT1 preferred polyunsaturated acyl CoAs, CrDGTT2 preferred monounsaturated acyl CoAs, and CrDGTT3 preferred C16 CoAs. When diacylglycerol was used as the substrate, CrDGTT1 preferred C16 over C18 in the sn-2 position of the glycerol backbone, but CrDGTT2 and CrDGTT3 preferred C18 over C16. In vivo knockdown of CrDGTT1, CrDGTT2 or CrDGTT3 resulted in 20-35% decreases in TAG content and a reduction of specific TAG fatty acids, in agreement with the findings of the invitro assay and fatty acid feeding test. These results demonstrate that CrDGTT1, CrDGTT2 and CrDGTT3 possess distinct specificities toward acyl CoAs and diacylglycerols, and may work in concert spatially and temporally to synthesize diverse TAG species in C.reinhardtii. CrDGTT1 was shown to prefer prokaryotic lipid substrates and probably resides in both the endoplasmic reticulum and chloroplast envelope, indicating its role in prokaryotic and eukaryotic TAG biosynthesis. Based on these findings, we propose a working model for the role of CrDGTT1 in TAG biosynthesis. This work provides insight into TAG biosynthesis in C.reinhardtii, and paves the way for engineering microalgae for production of biofuels and high-value bioproducts. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Plant Sciences |
| 研究领域[WOS] | Plant Sciences |
| 关键词[WOS] | FATTY-ACID SYNTHESIS ; ACYL-COA ; ENDOPLASMIC-RETICULUM ; NITROGEN DEPRIVATION ; LIPID-METABOLISM ; MEMBRANE-LIPIDS ; ACCUMULATION ; GENE ; ARABIDOPSIS ; CLONING |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000374980000001 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/27949]  |
| 专题 | 水生生物研究所_淡水生态学研究中心 |
| 作者单位 | 1.Univ Maryland, Ctr Environm Sci, Inst Marine & Environm Technol, Baltimore, MD 21202 USA 2.Univ Maryland Baltimore Cty, Baltimore, MD 21202 USA 3.Peking Univ, Coll Engn, Dept Energy & Resources Engn, Beijing 100871, Peoples R China 4.Chinese Acad Sci, Inst Hydrobiol, Ctr Microalgal Biotechnol & Biofuels, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Jin,Han, Danxiang,Yoon, Kangsup,et al. Characterization of type 2 diacylglycerol acyltransferases in Chlamydomonas reinhardtii reveals their distinct substrate specificities and functions in triacylglycerol biosynthesis[J]. PLANT JOURNAL,2016,86(1):3-19. |
| APA | Liu, Jin,Han, Danxiang,Yoon, Kangsup,Hu, Qiang,&Li, Yantao.(2016).Characterization of type 2 diacylglycerol acyltransferases in Chlamydomonas reinhardtii reveals their distinct substrate specificities and functions in triacylglycerol biosynthesis.PLANT JOURNAL,86(1),3-19. |
| MLA | Liu, Jin,et al."Characterization of type 2 diacylglycerol acyltransferases in Chlamydomonas reinhardtii reveals their distinct substrate specificities and functions in triacylglycerol biosynthesis".PLANT JOURNAL 86.1(2016):3-19. |
入库方式： OAI收割
来源：水生生物研究所
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