Optimization protocol for the extraction of antioxidant components from Origanum vulgare leaves using response surface methodology
文献类型：期刊论文
| 作者 | Majeed, Mudasir1; Hussain, Abdullah I.1; Chatha, Shahzad A. S.1; Khosa, Muhammad K. K.1; Kamal, Ghulam Mustafa2,3; Kamal, Mohammad A.4; Zhang, Xu2,3; Liu, Maili2,3 |
| 刊名 | SAUDI JOURNAL OF BIOLOGICAL SCIENCES
 |
| 出版日期 | 2016-05-01 |
| 卷号 | 23期号:3页码:389-396 |
| 关键词 | DPPH free radical scavenging activity Total phenolics Optimization Response surface methodology Central composite rotatable design Antioxidant compounds |
| 英文摘要 | In the present work, the response surface methodology (RSM) based on a central composite rotatable design (CCRD), was used to determine optimum conditions for the extraction of antioxidant compounds from Origanum vulgare leaves. Four process variables were evaluated at three levels (31 experimental designs): methanol (70%, 80%, and 90%), the solute:solvent ratio (1:5, 1:12.5, 1:20), the extraction time (4, 10, 16 h), and the solute particle size (20, 65, 110 micron). Using RSM, a quadratic polynomial equation was obtained by multiple regression analysis for predicting optimization of the extraction protocol. Analysis of variance (ANOVA) was applied and the significant effect of the factors and their interactions were tested at 95% confidence interval. The antioxidant extract (AE) yield was significantly influenced by solvent composition, solute to solvent ratio, and time. The maximum AE was obtained at methanol (70%), liquid solid ratio (20), time (16 h), and particle size (20 micron). Predicted values thus obtained were closer to the experimental value indicating suitability of the model. Run 25 (methanol:water 70:30; solute:solvent 1:20; extraction time 16 h and solute particle size 20) showed highest TP contents (18.75 mg/g of dry material, measured as gallic acid equivalents) and DPPH radical scavenging activity (IC50 5.04 mu g/mL). Results of the present study indicated good correlation between TP contents and DPPH radical scavenging activity. Results of the study indicated that phenolic compounds are powerful scavengers of free radical as demonstrated by a good correlation between TP contents and DPPH radical scavenging activity. (C) 2015 The Authors. Production and hosting by Elsevier B.V. on behalf of King Saud University. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biology |
| 研究领域[WOS] | Life Sciences & Biomedicine - Other Topics |
| 关键词[WOS] | ESSENTIAL OIL ; ANTIMICROBIAL ACTIVITIES ; CHEMICAL-COMPOSITION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000373377400011 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/9314]  |
| 专题 | 武汉物理与数学研究所_磁共振基础研究部 |
| 作者单位 | 1.Govt Coll Univ, Inst Chem, Faisalabad 38000, Pakistan 2.Chinese Acad Sci, Wuhan Inst Phys & Math, Ctr Magnet Resonance, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 10049, Peoples R China 4.King Abdalaziz Univ, King Fahad Med Res Ctr, Jeddah, Saudi Arabia |
| 推荐引用方式 GB/T 7714 | Majeed, Mudasir,Hussain, Abdullah I.,Chatha, Shahzad A. S.,et al. Optimization protocol for the extraction of antioxidant components from Origanum vulgare leaves using response surface methodology[J]. SAUDI JOURNAL OF BIOLOGICAL SCIENCES,2016,23(3):389-396. |
| APA | Majeed, Mudasir.,Hussain, Abdullah I..,Chatha, Shahzad A. S..,Khosa, Muhammad K. K..,Kamal, Ghulam Mustafa.,...&Liu, Maili.(2016).Optimization protocol for the extraction of antioxidant components from Origanum vulgare leaves using response surface methodology.SAUDI JOURNAL OF BIOLOGICAL SCIENCES,23(3),389-396. |
| MLA | Majeed, Mudasir,et al."Optimization protocol for the extraction of antioxidant components from Origanum vulgare leaves using response surface methodology".SAUDI JOURNAL OF BIOLOGICAL SCIENCES 23.3(2016):389-396. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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