Biothiol Xenon MRI Sensor Based on Thiol-Addition Reaction
文献类型：期刊论文
| 作者 | Yang, Shengjun1; Jiang, Weiping1; Ren, Lili1; Yuan, Yaping1; Zhang, Bin1; Luo, Qing1; Guo, Qianni1; Bouchard, Louis-S.2; Liu, Maili1; Zhou, Xin1 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2016-06-07 |
| 卷号 | 88期号:11页码:5835-5840 |
| 英文摘要 | Biothiols such as cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) play an important role in regulating the vital functions of living organisms. Knowledge of their biodistribution in real-time could help diagnose a variety of conditions. However, existing methods of biothiol detection are invasive and require assays. Herein we report a molecular biosensor for biothiol detection using the nuclear spin resonance of Xe-129. The Xe-129 biosensor consists of a cryptophane cage encapsulating a xenon atom and an acrylate group. The latter serves as a reactive site to covalently bond biothiols through a thiol-addition reaction. The biosensor enables discrimination of Cys from Hcy and GSH through the chemical shift and average reaction rate. This biosensor can be detected at a concentration of 10 mu M in a single scan and it has been applied to detect biothiols in bovine serum solution. Our results indicate that this biosensor is a promising tool for the real-time imaging of biothiol distributions. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | NUCLEAR-MAGNETIC-RESONANCE ; HUMAN CARBONIC-ANHYDRASE ; XE-129 NMR ; SELECTIVE DETECTION ; MASS-SPECTROMETRY ; LIVING CELLS ; IN-VIVO ; FLUORESCENCE DETECTION ; FUNCTIONALIZED XENON ; CYSTEINE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000377631000041 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/9406]  |
| 专题 | 武汉物理与数学研究所_磁共振基础研究部 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Magnet Resonance & Atom & Mol Phys, Key Lab Magnet Resonance Biol Syst, Natl Ctr Magnet Resonance Wuhan,Wuhan Inst Phys &, Wuhan 430071, Peoples R China 2.Univ Calif Los Angeles, Dept Chem & Biochem, Calif NanoSyst Inst, Inst Mol Biol, Los Angeles, CA 90095 USA |
| 推荐引用方式 GB/T 7714 | Yang, Shengjun,Jiang, Weiping,Ren, Lili,et al. Biothiol Xenon MRI Sensor Based on Thiol-Addition Reaction[J]. ANALYTICAL CHEMISTRY,2016,88(11):5835-5840. |
| APA | Yang, Shengjun.,Jiang, Weiping.,Ren, Lili.,Yuan, Yaping.,Zhang, Bin.,...&Zhou, Xin.(2016).Biothiol Xenon MRI Sensor Based on Thiol-Addition Reaction.ANALYTICAL CHEMISTRY,88(11),5835-5840. |
| MLA | Yang, Shengjun,et al."Biothiol Xenon MRI Sensor Based on Thiol-Addition Reaction".ANALYTICAL CHEMISTRY 88.11(2016):5835-5840. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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