Hemi-methylated DNA opens a closed conformation of UHRF1 to facilitate its histone recognition
文献类型：期刊论文
| 作者 | Fang, Jian1,2,3; Cheng, Jingdong1; Wang, Jiaolong4; Zhang, Qiao5,6; Liu, Mengjie1; Gong, Rui1; Wang, Ping1; Zhang, Xiaodan1; Feng, Yangyang1; Lan, Wenxian4 |
| 刊名 | NATURE COMMUNICATIONS
 |
| 出版日期 | 2016-04-01 |
| 卷号 | 7 |
| 英文摘要 | UHRF1 is an important epigenetic regulator for maintenance DNA methylation. UHRF1 recognizes hemi-methylated DNA (hm-DNA) and trimethylation of histone H3K9 (H3K9me3), but the regulatory mechanism remains unknown. Here we show that UHRF1 adopts a closed conformation, in which a C-terminal region (Spacer) binds to the tandem Tudor domain (TTD) and inhibits H3K9me3 recognition, whereas the SET-and-RING-associated (SRA) domain binds to the plant homeodomain (PHD) and inhibits H3R2 recognition. Hm-DNA impairs the intramolecular interactions and promotes H3K9me3 recognition by TTD-PHD. The Spacer also facilitates UHRF1-DNMT1 interaction and enhances hm-DNA-binding affinity of the SRA. When TTD-PHD binds to H3K9me3, SRA-Spacer may exist in a dynamic equilibrium: either recognizes hm-DNA or recruits DNMT1 to chromatin. Our study reveals the mechanism for regulation of H3K9me3 and hm-DNA recognition by URHF1. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | EPIGENETIC REGULATOR UHRF1 ; SRA DOMAIN ; MAMMALIAN DEVELOPMENT ; BINDING-PROTEIN ; GENE-EXPRESSION ; METHYLTRANSFERASE DNMT1 ; STRUCTURAL BASIS ; TANDEM TUDOR ; PHD FINGER ; H3 TAIL |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000373621000001 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/9316]  |
| 专题 | 武汉物理与数学研究所_磁共振应用研究部 |
| 作者单位 | 1.Fudan Univ, Shanghai Med Coll, Sch Basic Med Sci, Key Lab Mol Med,Minist Educ,Dept Syst Biol Med, Shanghai 200032, Peoples R China 2.Fudan Univ, Sch Life Sci, Collaborat Innovat Ctr Genet & Dev, State Key Lab Genet Engn, Shanghai 200433, Peoples R China 3.Fudan Univ, Shanghai Med Coll, Shanghai Canc Ctr, Inst Biomed Sci, Shanghai 200032, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Organ Chem, State Key Lab Bioorgan & Nat Prod Chem, 345 LingLing Rd, Shanghai 200032, Peoples R China 5.E China Normal Univ, Inst Biomed Sci, Shanghai Key Lab Regulatory Biol, Shanghai 200241, Peoples R China 6.E China Normal Univ, Sch Life Sci, Shanghai 200241, Peoples R China 7.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fang, Jian,Cheng, Jingdong,Wang, Jiaolong,et al. Hemi-methylated DNA opens a closed conformation of UHRF1 to facilitate its histone recognition[J]. NATURE COMMUNICATIONS,2016,7. |
| APA | Fang, Jian.,Cheng, Jingdong.,Wang, Jiaolong.,Zhang, Qiao.,Liu, Mengjie.,...&Xu, Yanhui.(2016).Hemi-methylated DNA opens a closed conformation of UHRF1 to facilitate its histone recognition.NATURE COMMUNICATIONS,7. |
| MLA | Fang, Jian,et al."Hemi-methylated DNA opens a closed conformation of UHRF1 to facilitate its histone recognition".NATURE COMMUNICATIONS 7(2016). |
入库方式： OAI收割
来源：武汉物理与数学研究所
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