A sensitive fluorescent biosensor for the detection of copper ion inspired by biological recognition element pyoverdine
文献类型:期刊论文
作者 | Yin, K; Wu, YX; Wang, SS; Chen, LX![]() |
刊名 | SENSORS AND ACTUATORS B-CHEMICAL
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出版日期 | 2016-09-01 |
卷号 | 232页码:257-263 |
关键词 | Copper ion Fluorescent biosensor Pyoverdine Electron transfer |
ISSN号 | 0925-4005 |
产权排序 | [Yin, Kun; Wu, Yixuan; Wang, Shasha; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat; [Yin, Kun; Wu, Yixuan; Wang, Shasha] Univ Chinese Acad Sci |
通讯作者 | Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China. lxchen@yic.ac.cn |
英文摘要 | The environmental copper pollution seriously threatens the health of organisms and the safety of ecosystem. Therefore, the development of a simple and sensitive method to detect copper ion is very important. In this study, we have developed a fluorescent biosensor based on biological recognition element pyoverdine to selectively detect copper ion. The fluorescence of pyoverdine is quenched obviously after binding with copper ion. A good linearity within the range of 0.2-10 mu M (R= 0.997) is attained and the detection limit is 50 nM. The biosensor has been successfully utilized for the detection of copper ion in drinking water, seawater and bio-samples and the results agree well with those obtained by the inductively coupled plasma mass spectrometry. Therefore, the established biosensor is a creditable method to detect copper ion with high sensitivity and selectivity, which can be utilized as a powerful tool to monitor copper pollution in the environment. (C) 2016 Elsevier B.V. All rights reserved. |
研究领域[WOS] | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
关键词[WOS] | SOL-GEL GLASS ; PSEUDOMONAS-AERUGINOSA ; COLORIMETRIC DETECTION ; SELECTIVE DETECTION ; LIVING CELLS ; SENSOR ; CU2+ ; SIDEROPHORE ; CHEMOSENSOR ; DISEASE |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000375483000032 |
源URL | [http://ir.yic.ac.cn/handle/133337/17075] ![]() |
专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat 2.Univ Chinese Acad Sci |
推荐引用方式 GB/T 7714 | Yin, K,Wu, YX,Wang, SS,et al. A sensitive fluorescent biosensor for the detection of copper ion inspired by biological recognition element pyoverdine[J]. SENSORS AND ACTUATORS B-CHEMICAL,2016,232:257-263. |
APA | Yin, K,Wu, YX,Wang, SS,&Chen, LX.(2016).A sensitive fluorescent biosensor for the detection of copper ion inspired by biological recognition element pyoverdine.SENSORS AND ACTUATORS B-CHEMICAL,232,257-263. |
MLA | Yin, K,et al."A sensitive fluorescent biosensor for the detection of copper ion inspired by biological recognition element pyoverdine".SENSORS AND ACTUATORS B-CHEMICAL 232(2016):257-263. |
入库方式: OAI收割
来源:烟台海岸带研究所
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