One-step chromatographic procedure for purification of B-phycoerythrin from Porphyridium cruentum
文献类型：期刊论文
| 作者 | Tang, ZH; Zhao, JL; Ju, B; Li, WJ ; Wen, SH; Pu, Y; Qin, S
|
| 刊名 | PROTEIN EXPRESSION AND PURIFICATION
 |
| 出版日期 | 2016-07-01 |
| 卷号 | 123页码:70-74 |
| 关键词 | Porphyridium cruentum B-phycoerythrin Purification Chromatography |
| ISSN号 | 1046-5928 |
| 产权排序 | [Tang, Zhihong; Zhao, Jilu; Ju, Bao; Wen, Shaohong] Yantai Univ, Coll Life Sci, 30 Qingquan St, Yantai 264005, Peoples R China; [Li, Wenjun; Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chunhui St, Yantai 264003, Peoples R China; [Pu, Yang] Ludong Univ, Coll Agr, 186 Hongqizhong St, Yantai 264025, Peoples R China |
| 通讯作者 | Tang, ZH (reprint author), Yantai Univ, Coll Life Sci, 30 Qingquan St, Yantai 264005, Peoples R China. zhhtang6@163.com |
| 英文摘要 | B-phycoerythrin (B-PE) was separated and purified from microalga Porphyridium cruentum using one-step chromatographic method. Phycobiliproteins in P. cruentum was extracted by osmotic shock and initially purified by ultrafiltration. Further purification was carried out with a SOURCE 15Q exchange column and analytical grade B-PE was obtained with a purity ratio (A(545)/A(280)) of 5.1 and a yield of 68.5%. It showed a double absorption peaks at 545 nm and 565 nm and a shoulder peak at 498 nm, and displayed a fluorescence emission maximum at 580 nm. The analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) showed a bulky band between 18 and 20 kDa which could be assigned to subunits alpha and beta and a low intensity band of 27 kDa assigned to gamma subunit. Our protocol provides attractive alternative to consider for the purification procedure to obtain analytical grade B-PE at commercial level. (C) 2016 Elsevier Inc. All rights reserved. |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 关键词[WOS] | BED ADSORPTION CHROMATOGRAPHY ; R-PHYCOERYTHRIN ; PHYCOBILIPROTEINS ; SEPARATION ; RECOVERY ; ALLOPHYCOCYANIN ; PHYCOBILISOMES ; PHYCOCYANIN ; EXTRACTION ; PROTEINS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000376221600010 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/17077]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 作者单位 | 1.Yantai Univ, Coll Life Sci 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res 3.Ludong Univ |
| 推荐引用方式 GB/T 7714 | Tang, ZH,Zhao, JL,Ju, B,et al. One-step chromatographic procedure for purification of B-phycoerythrin from Porphyridium cruentum[J]. PROTEIN EXPRESSION AND PURIFICATION,2016,123:70-74. |
| APA | Tang, ZH.,Zhao, JL.,Ju, B.,Li, WJ.,Wen, SH.,...&Qin, S.(2016).One-step chromatographic procedure for purification of B-phycoerythrin from Porphyridium cruentum.PROTEIN EXPRESSION AND PURIFICATION,123,70-74. |
| MLA | Tang, ZH,et al."One-step chromatographic procedure for purification of B-phycoerythrin from Porphyridium cruentum".PROTEIN EXPRESSION AND PURIFICATION 123(2016):70-74. |
入库方式： OAI收割
来源：烟台海岸带研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。