Development of a rapid assay to detect the jellyfish Cyanea nozakii using a loop-mediated isothermal amplification method
文献类型：期刊论文
| 作者 | Liu, ZY ; Dong, ZJ; Liu, DY
|
| 刊名 | MITOCHONDRIAL DNA
 |
| 出版日期 | 2016 |
| 卷号 | 27期号:4页码:2318-2322 |
| 关键词 | Cyanea nozakii cytochrome c oxidase subunit I jellyfish blooms LAMP mitochondrial DNA |
| ISSN号 | 1940-1736 |
| 产权排序 | [Liu, Zhongyuan; Dong, Zhijun; Liu, Dongyan] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai, Shandong, Peoples R China; [Liu, Zhongyuan] Univ Chinese Acad Sci, Beijing, Peoples R China |
| 通讯作者 | Dong, ZJ (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China. zjdong@yic.ac.cn |
| 英文摘要 | Blooms of the harmful jellyfish Cyanea nozakii, which are a severe nuisance to fisheries and tourisms, frequently occur in the northern East China Sea, Yellow Sea, and Bohai Sea. To provide early warning of this species, a simple and effective molecular method for identifying C. nozakii was developed using the loop-mediated isothermal amplification method (LAMP). The LAMP assay is highly specific and uses a set of four primers that target six different regions on the mitochondrial cytochrome c oxidase subunit I (COI) gene of C. nozakii. The amplification conditions, including the dNTP and betaine concentrations, the inner primer to outer primer concentration ratio, reaction time and temperature, were optimized. The LAMP assay amplified DNA extracted from tissue samples of C. nozakii but did not amplify DNA from other common scyphozoans and hydrozoans collected in the same region. In addition, the LAMP assay was more sensitive than conventional PCR. Therefore, the established LAMP assay is a sensitive, specific, fast, and easily performed method for detection of C. nozakii at different stages in their life cycle. |
| 研究领域[WOS] | Genetics & Heredity |
| 关键词[WOS] | SCYPHOZOAN JELLYFISH ; BLOOMS ; PCR ; SEQUENCES ; PRIMERS ; AURELIA ; POLYPS ; WATERS ; SEA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000377956300004 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/17090]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 烟台海岸带研究所_近岸生态与环境实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Remedia 2.Univ Chinese Acad Sci |
| 推荐引用方式 GB/T 7714 | Liu, ZY,Dong, ZJ,Liu, DY. Development of a rapid assay to detect the jellyfish Cyanea nozakii using a loop-mediated isothermal amplification method[J]. MITOCHONDRIAL DNA,2016,27(4):2318-2322. |
| APA | Liu, ZY,Dong, ZJ,&Liu, DY.(2016).Development of a rapid assay to detect the jellyfish Cyanea nozakii using a loop-mediated isothermal amplification method.MITOCHONDRIAL DNA,27(4),2318-2322. |
| MLA | Liu, ZY,et al."Development of a rapid assay to detect the jellyfish Cyanea nozakii using a loop-mediated isothermal amplification method".MITOCHONDRIAL DNA 27.4(2016):2318-2322. |
入库方式： OAI收割
来源：烟台海岸带研究所
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