Optimization and characterization of biosurfactant production from marine Vibrio sp strain 3B-2
文献类型:期刊论文
作者 | Hu, Xiaoke1![]() |
刊名 | FRONTIERS IN MICROBIOLOGY
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出版日期 | 2015-09-28 |
卷号 | 6 |
关键词 | microbial biosurfactant optimal production surface tension Vibrio alginolyticus glycoproteins |
ISSN号 | 1664-302X |
英文摘要 | A biosurfactant-producing bacterium, designated 3B-2, was isolated from marine sediment and identified as Vibrio sp. by 16S rRNA gene sequencing. The culture medium composition was optimized to increase the capability of 3B-2 for producing biosurfactant. The produced biosurfactant was characterized in terms of protein concentration, surface tension, and oil-displacement efficiency. The optimal medium for biosurfactant production contained: 0.5% lactose, 1.1% yeast extract, 2% sodium chloride, and 0.1% disodium hydrogen phosphate. Under optimal conditions (28 degrees C), the surface tension of crude biosurfactant could be reduced to 41 from 71.5 mN/m (water), while its protein concentration was increased to up to 6.5 g/L and the oil displacement efficiency was improved dramatically at 6.5 cm. Two glycoprotein fractions with the molecular masses of 22 and 40 kDa were purified from the biosurfactant, which held great potential for applications in microbial enhanced oil recovery and bioremediation. |
研究领域[WOS] | Microbiology |
关键词[WOS] | ANALYTICAL HIERARCHY PROCESS ; CRUDE-OIL ; BIOEMULSIFIER ; HYDROCARBON ; BACTERIA ; MICROORGANISMS ; FERMENTATION ; SURFACTANTS ; CULTURE ; FIBERS |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000378319700001 |
源URL | [http://ir.yic.ac.cn/handle/133337/17091] ![]() |
专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Chunhui Rd, Yantai 264003, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Ocean Univ China, Qingdao, Peoples R China |
推荐引用方式 GB/T 7714 | Hu, Xiaoke,Wang, Caixia,Wang, Peng. Optimization and characterization of biosurfactant production from marine Vibrio sp strain 3B-2[J]. FRONTIERS IN MICROBIOLOGY,2015,6. |
APA | Hu, Xiaoke,Wang, Caixia,&Wang, Peng.(2015).Optimization and characterization of biosurfactant production from marine Vibrio sp strain 3B-2.FRONTIERS IN MICROBIOLOGY,6. |
MLA | Hu, Xiaoke,et al."Optimization and characterization of biosurfactant production from marine Vibrio sp strain 3B-2".FRONTIERS IN MICROBIOLOGY 6(2015). |
入库方式: OAI收割
来源:烟台海岸带研究所
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