Determination of Histamine by High-Performance Liquid Chromatography After Precolumn Derivatization with o-Phthalaldehyde-Sulfite
文献类型：期刊论文
| 作者 | Chen, Rongxiang1,2; Deng, Yinghua3; Yang, Liu3; Wang, Jie2; Xu, Fuqiang2,4 |
| 刊名 | JOURNAL OF CHROMATOGRAPHIC SCIENCE
 |
| 出版日期 | 2016-04-01 |
| 卷号 | 54期号:4页码:547-553 |
| 英文摘要 | A fast and sensitive method was developed for in vivo determination of histamine in the brain microdialysate by reverse ion pair chromatography with electrochemical detection. The microdialysates were derivatized with o-phthalaldehyde and sodium sulfite, and separation was achieved using isocratic elution within 10 min. The separation was performed in an Agilent Eclipse Plus C18 column (3.0 x 150 mm, particle size 3.5 mu m), and the mobile phase consisted of 100 mM monosodium phosphate (pH 6.0), 500 mg L-1 OSA and 20% methanol (v/v). The linearity (R-2) was found to be >0.999, with a range from 2 to 50 nM and excellent repeatability (relative standard deviation, 2.29-6.04%), and the limit of detection was 0.4 nM. This method was successfully applied to analyze the extracellular concentration of histamine in the hypothalamus of rats, with probe recovery calculated in vivo. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | ELECTROCHEMICAL DETECTION ; FLUORESCENCE DETECTION ; MICRODIALYSIS SAMPLES ; BIOLOGICAL SAMPLES ; BRAIN HISTAMINE ; RAT-BRAIN ; IN-VITRO ; NEUROTRANSMITTERS ; QUANTITATION ; GLUTAMATE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000380263900009 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/9479]  |
| 专题 | 武汉物理与数学研究所_磁共振应用研究部 |
| 作者单位 | 1.Zunyi Med Univ, Res Ctr Med & Biol, Zunyi 563003, Guizhou, Peoples R China 2.Chinese Acad Sci, State Key Lab Magnet Resonance & Atom & Mol Phys, Key Lab Magnet Resonance Biol Syst, Wuhan Inst Phys & Math, Wuhan 430071, Hubei, Peoples R China 3.Hubei Univ Educ, Dept Chem & Life Sci, Wuhan 430205, Hubei, Peoples R China 4.Wuhan Natl Lab Optoelect, Wuhan 430074, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Rongxiang,Deng, Yinghua,Yang, Liu,et al. Determination of Histamine by High-Performance Liquid Chromatography After Precolumn Derivatization with o-Phthalaldehyde-Sulfite[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE,2016,54(4):547-553. |
| APA | Chen, Rongxiang,Deng, Yinghua,Yang, Liu,Wang, Jie,&Xu, Fuqiang.(2016).Determination of Histamine by High-Performance Liquid Chromatography After Precolumn Derivatization with o-Phthalaldehyde-Sulfite.JOURNAL OF CHROMATOGRAPHIC SCIENCE,54(4),547-553. |
| MLA | Chen, Rongxiang,et al."Determination of Histamine by High-Performance Liquid Chromatography After Precolumn Derivatization with o-Phthalaldehyde-Sulfite".JOURNAL OF CHROMATOGRAPHIC SCIENCE 54.4(2016):547-553. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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