Invertase-mediated system for simple and rapid detection of pathogen
文献类型：期刊论文
| 作者 | Wan, Yi; Qi, Peng ; Zeng, Yan; Sun, Yan; Zhang, Dun
|
| 刊名 | SENSORS AND ACTUATORS B-CHEMICAL
 |
| 出版日期 | 2016-10-05 |
| 卷号 | 233页码:454-458 |
| 关键词 | Commercial glucose meter Bacteria detection Invertase Aptamer |
| 英文摘要 | Portable, low-cost and quantitative analysis of pathogen at home and in the field remain a key challenge in medical diagnostics and food safety. Despite many years of research, very few such tools are available. Motivated by present clinical requirements, pocket-sized device, such as commercial glucose meter (CGM), have emerged. Taking advantage of the CGM, we develop an approach to quantify bacteria. Molecular recognition elements, such as aptamer, coupled with a signal amplification method based on invertase-promoted hydrolysis of sucrose for the sensitive and selective detection of bacteria. This paper aims to develop a portable device combined with enzyme-mediated enhancement for biological/chemical analyte detection. A linear relationship between the response and the logarithm of the Staphylococcus aureus concentration using this method was obtained for concentrations ranging from 1.2 x 10(5) to 1.2 x 10(8) cfu mL(-1), with a detection limit of 1.0 x 10(5) cfu mL(-1). This study demonstrates an approach for bacteria detection and also provides a powerful platform to monitor bacterial infection, thus rendering potential broad antibacterial applications and bacteria diagnosis. (C) 2016 Elsevier B.V. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000376384100034 |
| 版本 | 出版稿 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/131084]  |
| 专题 | 海洋研究所_海洋腐蚀与防护研究发展中心 |
| 作者单位 | Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wan, Yi,Qi, Peng,Zeng, Yan,et al. Invertase-mediated system for simple and rapid detection of pathogen[J]. SENSORS AND ACTUATORS B-CHEMICAL,2016,233:454-458. |
| APA | Wan, Yi,Qi, Peng,Zeng, Yan,Sun, Yan,&Zhang, Dun.(2016).Invertase-mediated system for simple and rapid detection of pathogen.SENSORS AND ACTUATORS B-CHEMICAL,233,454-458. |
| MLA | Wan, Yi,et al."Invertase-mediated system for simple and rapid detection of pathogen".SENSORS AND ACTUATORS B-CHEMICAL 233(2016):454-458. |
入库方式： OAI收割
来源：海洋研究所
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