Conserved elements in the nanos3 3 ' UTR of olive flounder are responsible for the selective retention of RNA in germ cells
文献类型：期刊论文
| 作者 | Li, Meijie1,2,3 ; Tan, Xungang1,3; Sui, Yulei1,2,3; Jiao, Shuang1,3; Wu, Zhihao1,3; You, Feng1,3
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| 刊名 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
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| 出版日期 | 2016-08-01 |
| 卷号 | 198页码:66-72 |
| 关键词 | Chimeric RNA GCAC site Germ plasm Microinjection U-rich regions |
| 英文摘要 | In teleost fish, primordial germ cells (PGCs) are specified very early during embryogenesis and migrate to the site that gonads are formed. A previous study indicated that nanos3 is specifically expressed in PGCs, and the 3'untranslated region (UTR) of nanos3 is responsible for the localization of mRNA in these cells. In this study, we aimed to investigate the functional regions of nanos3 3'UTR in olive flounder using truncated and mutated nanos3 3'UTRs fused to chimeric RNAs and microinjected into fertilized zebrafish eggs. The results indicated that a 68-bp functional element in the nanos3 3'UTR of olive flounder played important roles in the protection and degradation of RNA. Within this element, a U-rich region was identified to be responsible for the protection of RNA in PGCs and two GCAC sites for the degradation of RNA in somatic cells. The first GCAC was located adjacently to the U-rich region and the second GCAC within the U-rich region. Overall, we concluded that the two GCACs were the binding sites of miR-430, a microRNA that suppresses translation, whereas the U-rich region was the binding site of Dnd, a protein that antagonizes the miR-430-mediated silencing of mRNA. (C)C. 2016 Elsevier Inc All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000378182300008 |
| 版本 | 出版稿 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/131000]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Meijie,Tan, Xungang,Sui, Yulei,et al. Conserved elements in the nanos3 3 ' UTR of olive flounder are responsible for the selective retention of RNA in germ cells[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2016,198:66-72. |
| APA | Li, Meijie,Tan, Xungang,Sui, Yulei,Jiao, Shuang,Wu, Zhihao,&You, Feng.(2016).Conserved elements in the nanos3 3 ' UTR of olive flounder are responsible for the selective retention of RNA in germ cells.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,198,66-72. |
| MLA | Li, Meijie,et al."Conserved elements in the nanos3 3 ' UTR of olive flounder are responsible for the selective retention of RNA in germ cells".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 198(2016):66-72. |
入库方式： OAI收割
来源：海洋研究所
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