Production of crocetin in transgenic Chlorella vulgaris expressing genes crtRB and ZCD1
文献类型：期刊论文
| 作者 | Lou, Sulin1; Wang, Liuying1; He, Lijuan1; Wang, Zhaokai1; Wang, Guangce2,3; Lin, Xiangzhi1 |
| 刊名 | JOURNAL OF APPLIED PHYCOLOGY
 |
| 出版日期 | 2016-06-01 |
| 卷号 | 28期号:3页码:1657-1665 |
| 关键词 | Chlorella vulgaris crtRB ZCD1 Genetic engineering Crocetin |
| 英文摘要 | Crocetin is one of the major active ingredients of the rare medicinal herb saffron (Crocus sativus L.), also known as crocus. Crocetin has a wide range of effects including antitumor, anticancer, antioxidant, and antiatherosclerotic activity. Crocetin is currently only obtained via extraction from crocus stigmas, with limited yields. This study developed a new method of production of crocetin from genetically engineered Chlorella vulgaris, a unicellular, fast-growing, and heterotrophically cultured green microalga. A plant expression vector carrying the genes crtRB and ZCD1, which encode enzymes controlling critical steps in crocetin biosynthesis, was introduced into C. vulgaris using the Agrobacterium tumefaciens-mediated transformation method. After hygromycin screening, resistant C. vulgaris strains were achieved. Polymerase chain reaction amplification confirmed that the crtRB and ZCD1 were successfully integrated into the C. vulgaris genome. High-performance liquid chromatography analysis of methanol extraction showed that the transgenic C. vulgaris was able to produce crocetin, while wild-type C. vulgaris was not. These results strongly suggest that introducing crtRB and ZCD1 into C. vulgaris can successfully produce crocetin. This is a new method of crocetin production in C. vulgaris to compensate for the shortage of crocetin. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000375315100021 |
| 版本 | 出版稿 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/131065]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.State Ocean Adm, Engn Res Ctr Marine Biol Resource Comprehens Util, Inst Oceanog 3, 184 Daxue Rd, Xiamen 361005, Fujian Province, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Nanhai Rd 7, Qingdao, Shandong, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lou, Sulin,Wang, Liuying,He, Lijuan,et al. Production of crocetin in transgenic Chlorella vulgaris expressing genes crtRB and ZCD1[J]. JOURNAL OF APPLIED PHYCOLOGY,2016,28(3):1657-1665. |
| APA | Lou, Sulin,Wang, Liuying,He, Lijuan,Wang, Zhaokai,Wang, Guangce,&Lin, Xiangzhi.(2016).Production of crocetin in transgenic Chlorella vulgaris expressing genes crtRB and ZCD1.JOURNAL OF APPLIED PHYCOLOGY,28(3),1657-1665. |
| MLA | Lou, Sulin,et al."Production of crocetin in transgenic Chlorella vulgaris expressing genes crtRB and ZCD1".JOURNAL OF APPLIED PHYCOLOGY 28.3(2016):1657-1665. |
入库方式： OAI收割
来源：海洋研究所
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