background: the bacterium acetobacter sp. cctcc m209061 is a promising whole-cell biocatalyst with exclusive anti-prelog stereoselectivity for the reduction of prochiral ketones that can be used to make valuable chiral alcohols such as (r)-4-(trimethylsilyl)-3-butyn-2-ol. although it has promising catalytic properties, its stability and reusability are relatively poor compared to other biocatalysts. hence, we explored various materials for immobilizing the active cells, in order to improve the operational stability of biocatalyst.