Experimental study of continuous-flow polymerase chain reaction microfluidics based on polytetrafluoroethylene capillary
文献类型：期刊论文
| 作者 | Zhang Chunsun; Xu Jinliang; Wang Jianqin; Wang Hanping |
| 刊名 | chinese journal of analytical chemistry
 |
| 出版日期 | 2006-08-01 |
| 卷号 | 34期号:8页码:1197-1202 |
| 关键词 | capillary continuous-flow polymerase chain reaction microfluidics |
| 英文摘要 | the polytetrafluoroethylene ( ptfe ) capillary-based continuous-flow polymerase chain reaction ( pcr ) microfluidics was constructed by using the flexible thin film heaters to form three constant temperature zones necessary for a pcr amplification, in which the pcr mixture could flow through the capillary microchannel continuously, thus leading,to the successful completion of pcr exponential amplification. the effects of various concentrations of bovine serum albumin ( bsa ) in a 25 mu l pcr mixture and flow rates of pcr mixture in the capillary microchannel, on continuous-flow pcr amplification, had been discussed in detail, where a pcr amplification of 249 base pair (bp) human beta-actin gene fragments was successfully performed within 15 and even 10 minutes, which is several times faster than the reaction time required ( 1 - 2 h) with the conventional pcr machine. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | dna amplification ; pcr chip ; device ; polycarbonate |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000243165100036 |
| 源URL | [http://ir.giec.ac.cn/handle/344007/10263]  |
| 专题 | 中国科学院广州能源研究所 |
| 作者单位 | 1.Chinese Acad Sci, Micro Energy Syst Lab, Guangzhou Inst Energy Convers, Guangzhou 510640, Peoples R China 2.Univ Sci & Technol China, Dept Thermal Sci & Energy Engn, Anhua 230026, Peoples R China 3.Guangzhou First Municipal Peoples Hosp, Guangzhou 510180, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang Chunsun,Xu Jinliang,Wang Jianqin,et al. Experimental study of continuous-flow polymerase chain reaction microfluidics based on polytetrafluoroethylene capillary[J]. chinese journal of analytical chemistry,2006,34(8):1197-1202. |
| APA | Zhang Chunsun,Xu Jinliang,Wang Jianqin,&Wang Hanping.(2006).Experimental study of continuous-flow polymerase chain reaction microfluidics based on polytetrafluoroethylene capillary.chinese journal of analytical chemistry,34(8),1197-1202. |
| MLA | Zhang Chunsun,et al."Experimental study of continuous-flow polymerase chain reaction microfluidics based on polytetrafluoroethylene capillary".chinese journal of analytical chemistry 34.8(2006):1197-1202. |
入库方式： OAI收割
来源：广州能源研究所
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