Continuous-flow PCR microfluidics for rapid DNA amplification using thin film heater with low thermal mass
文献类型：期刊论文
| 作者 | Zhang, C.; Xing, D.; Xu, J. |
| 刊名 | analytical letters
 |
| 出版日期 | 2007 |
| 卷号 | 40期号:9页码:1672-1685 |
| 关键词 | continuous-flow polymerase chain reaction (PCR) microfluidics DNA amplification thin film heater |
| 英文摘要 | we have described a compact capillary-based continuous-flow polymerase chain reaction ( pcr) microfluidics device, which uses flexible thin film heaters with low thermal mass to construct three isothermal zones. due to the decreased thermal mass of flexible thin film heater, the low power supply and rapid thermal response was obtained. the energy consumption of a 33-cycle continuous-flow pcr was less than 0.0088 kw h, which is much lower than that of the metal block or liquid bath based capillary continuous-flow pcr microfluidics. special attention was also paid on the surface passivation of the capillary inner surface based on the competing bovine serum albumin ( bsa), and the results showed the effect of dynamic passivation was superior to that of static passivation. with the help of the dynamic passivation, the 249 bp human beta-actin gene fragment was amplified in 15 min, which is several times faster than that of the conventional pcr machine. in addition, the effect of initial dna template concentrations on continuous-flow pcr was also investigated. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | polymerase-chain-reaction ; chip |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000247761900002 |
| 源URL | [http://ir.giec.ac.cn/handle/344007/10275]  |
| 专题 | 中国科学院广州能源研究所 |
| 作者单位 | 1.S China Normal Univ, MOE Key Lib Laser Life Sci, Guangzhou 510631, Peoples R China 2.S China Normal Univ, Inst Laser Life Sci, Guangzhou 510631, Peoples R China 3.Chinese Acad Sci, Micro Energy Syst Lab, Guangzhou Inst Energy Convers, Guangzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, C.,Xing, D.,Xu, J.. Continuous-flow PCR microfluidics for rapid DNA amplification using thin film heater with low thermal mass[J]. analytical letters,2007,40(9):1672-1685. |
| APA | Zhang, C.,Xing, D.,&Xu, J..(2007).Continuous-flow PCR microfluidics for rapid DNA amplification using thin film heater with low thermal mass.analytical letters,40(9),1672-1685. |
| MLA | Zhang, C.,et al."Continuous-flow PCR microfluidics for rapid DNA amplification using thin film heater with low thermal mass".analytical letters 40.9(2007):1672-1685. |
入库方式： OAI收割
来源：广州能源研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。