Characterization of direct cellulase immobilization with superparamagnetic nanoparticles
文献类型：期刊论文
| 作者 | Xu, Jingliang1; Huo, Shuhao2; Yuan, Zhenhong1; Zhang, Yu1; Xu, Huijuan1; Guo, Ying1; Liang, Cuiyi1; Zhuang, Xinshu1 |
| 刊名 | biocatalysis and biotransformation
 |
| 出版日期 | 2011-03-01 |
| 卷号 | 29期号:2-3页码:71-76 |
| 关键词 | Cellulase direct immobilization superparamagnetic nanoparticles glutaraldehyde |
| 英文摘要 | methods of cellulase immobilization on magnetic particles via glutaraldehyde binding were studied. the binding was confirmed by transmission electronic microscopy (tem), x-ray diffraction (xrd), fourier-transform infrared spectroscopy (ftir) and vibrating sample magnetometry (vsm). samples analyzed by tem and xrd showed that the magnetic particles with or without bound cellulase were all nanosized particles with a mean diameter of 11.5 nm, and the binding process did not cause significant changes in particle size and structure. analysis by ftir showed that the binding of cellulase to the magnetic nanoparticles might be via covalent bonding between residual amine groups on fe(3)o(4) nanoparticles and amine groups of the cellulase. the vsm analysis showed that magnetic nanoparticles with or without bound cellulase were all superparamagnetic. the immobilized cellulase had a wider ph and temperature range and improved storage stability compared with the free enzyme. determination of the michaelis constants revealed that the immobilized cellulase had a greater affinity for the cellulosic substrate than the free enzyme. the immobilized cellulase showed better performance on hydrolysis of steam-exploded corn stalks than of bleached sulfite bagasse pulp. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemistry & molecular biology ; biotechnology & applied microbiology |
| 研究领域[WOS] | biochemistry & molecular biology ; biotechnology & applied microbiology |
| 关键词[WOS] | direct binding procedure ; magnetic nanoparticles ; particles ; dehydrogenase ; stability |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000290868200004 |
| 源URL | [http://ir.giec.ac.cn/handle/344007/10413]  |
| 专题 | 中国科学院广州能源研究所 |
| 作者单位 | 1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Key Lab Renewable Energy & Gas Hydrate, Guangzhou 510640, Guangdong, Peoples R China 2.Capital Normal Univ, Coll Life Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Jingliang,Huo, Shuhao,Yuan, Zhenhong,et al. Characterization of direct cellulase immobilization with superparamagnetic nanoparticles[J]. biocatalysis and biotransformation,2011,29(2-3):71-76. |
| APA | Xu, Jingliang.,Huo, Shuhao.,Yuan, Zhenhong.,Zhang, Yu.,Xu, Huijuan.,...&Zhuang, Xinshu.(2011).Characterization of direct cellulase immobilization with superparamagnetic nanoparticles.biocatalysis and biotransformation,29(2-3),71-76. |
| MLA | Xu, Jingliang,et al."Characterization of direct cellulase immobilization with superparamagnetic nanoparticles".biocatalysis and biotransformation 29.2-3(2011):71-76. |
入库方式： OAI收割
来源：广州能源研究所
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