L-Valine production with minimization of by-products' synthesis in Corynebacterium glutamicum and Brevibacterium flavum
文献类型：期刊论文
| 作者 | Hou, Xiaohu1,2,3; Chen, Xinde1,2 ; Zhang, Yue3; Qian, He3; Zhang, Weiguo3
|
| 刊名 | amino acids
 |
| 出版日期 | 2012-12-01 |
| 卷号 | 43期号:6页码:2301-2311 |
| 关键词 | L-Valine Corynebacterium glutamicum Brevibacterium flavum alaT avtA |
| 英文摘要 | corynebacterium glutamicum atcc13032 and brevibacterium flavum jv16 were engineered for l-valine production by over-expressing ilvebn(r)c genes at 31 degrees c in 72 h fermentation. different strategies were carried out to reduce the by-products' accumulation in l-valine fermentation and also to increase the availability of precursor for l-valine biosynthesis. the native promoter of ilva of c. glutamicum was replaced with a weak promoter mpilva (p-ilvam1cg) to reduce the biosynthetic rate of l-isoleucine. effect of different relative dissolved oxygen on l-valine production and by-products' formation was recorded, indicating that 15 % saturation may be the most appropriate relative dissolved oxygen for l-valine fermentation with almost no l-lactic acid and l-glutamate formed. to minimize l-alanine accumulation, alat and/or avta was inactivated in c. glutamicum and b. flavum, respectively. compared to high concentration of l-alanine accumulated by alat inactivated strains harboring ilvebn(r)c genes, l-alanine concentration was reduced to 0.18 g/l by c. glutamicum atcc13032mpilva(delta)avta pdxw-8-ilvebn(r)c, and 0.22 g/l by b. flavum jv16avta::cm pdxw-8-ilvebn(r)c. meanwhile, l-valine production and conversion efficiency were enhanced to 31.15 g/l and 0.173 g/g by c. glutamicum atcc13032mpilva(delta)avta pdxw-8-ilvebn(r)c, 38.82 g/l and 0.252 g/g by b. flavum jv16avta::cm pdxw-8-ilvebn(r)c. this study provides combined strategies to improve l-valine yield by minimization of by-products' production. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemistry & molecular biology |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | escherichia-coli ; amino-acids ; metabolism ; pathway ; genes |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000310885500008 |
| 源URL | [http://ir.giec.ac.cn/handle/344007/10563]  |
| 专题 | 中国科学院广州能源研究所 集成技术研发中心 |
| 作者单位 | 1.Chinese Acad Sci, Lab Renewable Energy & Gas Hydrate, Guangzhou 510640, Guangdong, Peoples R China 2.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China 3.JiangNan Univ, Key Lab Ind Biotechnol, Minist Educ, Sch Biotechnol, Wuxi 214122, Jiangsu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hou, Xiaohu,Chen, Xinde,Zhang, Yue,et al. L-Valine production with minimization of by-products' synthesis in Corynebacterium glutamicum and Brevibacterium flavum[J]. amino acids,2012,43(6):2301-2311. |
| APA | Hou, Xiaohu,Chen, Xinde,Zhang, Yue,Qian, He,&Zhang, Weiguo.(2012).L-Valine production with minimization of by-products' synthesis in Corynebacterium glutamicum and Brevibacterium flavum.amino acids,43(6),2301-2311. |
| MLA | Hou, Xiaohu,et al."L-Valine production with minimization of by-products' synthesis in Corynebacterium glutamicum and Brevibacterium flavum".amino acids 43.6(2012):2301-2311. |
入库方式： OAI收割
来源：广州能源研究所
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