Evaluating the influence of inhibitors present in lignocellulosic hydrolysates on the cell membrane integrity of oleaginous yeast Trichosporon fermentans by flow cytometry
文献类型：期刊论文
| 作者 | Huang, Chao1,3,4; Zhu, Ding-he2; Wu, Hong1,2; Lou, Wen-yong1; Zong, Min-hua1 |
| 刊名 | process biochemistry
 |
| 出版日期 | 2014-03-01 |
| 卷号 | 49期号:3页码:395-401 |
| 关键词 | Trichosporon fermentans Organic acid Aldehyde Cell membrane integrity Flow cytometry Lignocellulosic hydrolysate |
| 英文摘要 | the effect of ten typical organic acids and five aldehydes present in lignocellulosic hydrolysates on the cell membrane integrity of oleaginous yeast trichosporon fermentans was evaluated by flow cytometry. overall, organic acids affected the cell membrane integrity of t. fermentans more significantly than that of aldehydes albeit aldehydes are more toxic to t. fermentans. the pi (propidium iodide) uptake rate of t. fermentans' cells gradually decreased as fermentation going on, indicating that t. fermentans could overcome the inhibition of organic acids or aldehydes by adaption. interestingly, in some cases, the effect of organic acids or aldehydes on the cell membrane integrity of t. fermentans was well related to their hydrophobicity. however, for the outliers, no obvious similar phenomena were observed. thus, the attack on hydrophobic sites of cell membrane was not the only determinant for the damage of organic acids or aldehydes on cell membrane integrity of t. fermentans. (c) 2013 elsevier ltd. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; technology |
| 类目[WOS] | biochemistry & molecular biology ; biotechnology & applied microbiology ; engineering, chemical |
| 研究领域[WOS] | biochemistry & molecular biology ; biotechnology & applied microbiology ; engineering |
| 关键词[WOS] | ethanologenic escherichia-coli ; saccharomyces-cerevisiae ; lipid-accumulation ; oil production ; organic-acids ; growth ; conversion ; aldehydes |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000334482300006 |
| 源URL | [http://ir.giec.ac.cn/handle/344007/10708]  |
| 专题 | 中国科学院广州能源研究所 |
| 作者单位 | 1.S China Univ Technol, State Key Lab Pulp & Paper Engn, Coll Light Ind & Food Sci, Guangzhou 510640, Guangdong, Peoples R China 2.S China Univ Technol, Prov Key Lab Green Proc Technol & Prod Safety Nat, Coll Light Ind & Food Sci, Guangzhou 510640, Guangdong, Peoples R China 3.Chinese Acad Sci, Key Lab Renewable Energy, Guangzhou 510640, Peoples R China 4.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Chao,Zhu, Ding-he,Wu, Hong,et al. Evaluating the influence of inhibitors present in lignocellulosic hydrolysates on the cell membrane integrity of oleaginous yeast Trichosporon fermentans by flow cytometry[J]. process biochemistry,2014,49(3):395-401. |
| APA | Huang, Chao,Zhu, Ding-he,Wu, Hong,Lou, Wen-yong,&Zong, Min-hua.(2014).Evaluating the influence of inhibitors present in lignocellulosic hydrolysates on the cell membrane integrity of oleaginous yeast Trichosporon fermentans by flow cytometry.process biochemistry,49(3),395-401. |
| MLA | Huang, Chao,et al."Evaluating the influence of inhibitors present in lignocellulosic hydrolysates on the cell membrane integrity of oleaginous yeast Trichosporon fermentans by flow cytometry".process biochemistry 49.3(2014):395-401. |
入库方式： OAI收割
来源：广州能源研究所
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