Functional roles of CymA and NapC in reduction of nitrate and nitrite by Shewanella putrefaciens W3-18-1
文献类型：期刊论文
| 作者 | Wei, Hehong1,2; Dai, Jingcheng1; Xia, Ming1,2; Romine, Margaret F.3; Shi, Liang3; Beliav, Alex3; Tiedje, James M.4; Nealson, Kenneth H.5; Fredrickson, James K.3; Zhou, Jizhong6,7 |
| 刊名 | MICROBIOLOGY-SGM
 |
| 出版日期 | 2016-06-01 |
| 卷号 | 162页码:930-941 |
| ISSN号 | 1350-0872 |
| 英文摘要 | Shewanella putrefaciens W3-18-1 harbours two periplasmic nitrate reductase (Nap) gene clusters, NapC-associated nap-alpha (napEDABC) and CymA-dependent nap-beta (napDAGHB), for dissimilatory nitrate respiration. CymA is a member of the NapC/NirT quinol dehydrogenase family and acts as a hub to support different respiratory pathways, including those on iron [Fe(III)] and manganese [Mn(III, IV)] (hydr)oxide, nitrate, nitrite, fumarate and arsenate in Shewanella strains. However, in our analysis it was shown that another NapC/NirT family protein, NapC, was only involved in nitrate reduction, although both CymA and NapC can transfer quinol-derived electrons to a periplasmic terminal reductase or an electron acceptor. Furthermore, our results showed that NapC could only interact specifically with the Nap-alpha nitrate reductase while CymA could interact promiscuously with Nap-alpha, Nap-beta and the NrfA nitrite reductase for nitrate and nitrite reduction. To further explore the difference in specificity, site-directed mutagenesis on both CymA and NapC was conducted and the phenotypic changes in nitrate and nitrite reduction were tested. Our analyses demonstrated that the Lys-91 residue played a key role in nitrate reduction for quinol oxidation and the Asp-166 residue might influence the maturation of CymA. The Asp-97 residue might be one of the key factors that influence the interaction of CymA with the cytochromes NapB and NrfA. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Microbiology |
| 研究领域[WOS] | Microbiology |
| 关键词[WOS] | RANGE CLONING VECTOR ; C-TYPE CYTOCHROME ; ONEIDENSIS MR-1 ; ELECTRON-TRANSPORT ; RESPIRATORY FLEXIBILITY ; POLYACRYLAMIDE GELS ; ESCHERICHIA-COLI ; CYCLIC-AMP ; GENE ; PROTEIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000382102900003 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/28383]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_学位论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Pacific Northwest Natl Lab, Richland, WA 99354 USA 4.Michigan State Univ, Ctr Microbial Ecol, E Lansing, MI 48824 USA 5.Univ So Calif, Dept Earth Sci, Los Angeles, CA 90089 USA 6.Univ Oklahoma, Inst Environm Genom, Norman, OK 73019 USA 7.Univ Oklahoma, Dept Plant Biol & Microbiol, Norman, OK 73019 USA |
| 推荐引用方式 GB/T 7714 | Wei, Hehong,Dai, Jingcheng,Xia, Ming,et al. Functional roles of CymA and NapC in reduction of nitrate and nitrite by Shewanella putrefaciens W3-18-1[J]. MICROBIOLOGY-SGM,2016,162:930-941. |
| APA | Wei, Hehong.,Dai, Jingcheng.,Xia, Ming.,Romine, Margaret F..,Shi, Liang.,...&Qiu, Dongru.(2016).Functional roles of CymA and NapC in reduction of nitrate and nitrite by Shewanella putrefaciens W3-18-1.MICROBIOLOGY-SGM,162,930-941. |
| MLA | Wei, Hehong,et al."Functional roles of CymA and NapC in reduction of nitrate and nitrite by Shewanella putrefaciens W3-18-1".MICROBIOLOGY-SGM 162(2016):930-941. |
入库方式： OAI收割
来源：水生生物研究所
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