Effects of pyrogallic acid on Microcystis aeruginosa: oxidative stress related toxicity
文献类型：期刊论文
| 作者 | Lu, Zhiying1,2; Zhang, Yongyuan1; Gao, Yunni1,3; Liu, Biyun1; Sun, Xuemei1,2; He, Feng1; Zhou, Qiaohong1; Wu, Zhenbin1 |
| 刊名 | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
 |
| 出版日期 | 2016-10-01 |
| 卷号 | 132页码:413-419 |
| 关键词 | Allelochemical Cause-effect relationships DNA strand breaks Microcystis aeruginosa Pyrogallic acid |
| ISSN号 | 0147-6513 |
| 英文摘要 | Pyrogallic acid (PA) is used in various industrial and consumer products. The molecular mechanisms underlying PA's toxicity was not fully understood. In this study, toxicity of PA on Microcystis aeruginosa with reactive oxygen species (ROS) generation as an end point was investigated. The results showed an increase in the percentage of cells with loss of membrane integrity and enhanced intracellular ROS production. Exposure to 50 mg L-1 PA for 48 h caused the highest percentage of loss of membrane integrity (56.7%), and a 2.54-fold higher intracellular ROS level compared to control. Further investigation revealed that PA caused a dose-dependent increase in DNA strand breaks (DSB) of M. aeruginosa at exposure concentration from 2 to 50 mg L-1. The incubation of cells with ROS scavengers ascorbic acid, N-acetyl-L-cysteine (NAC) and tocopherol markedly alleviated the level of PA-induced DSB. Analysis of PA autoxidized products in culture solution showed that PA was quickly converted to purpurogallin (PG), and PG was further autoxidized to other polyphenolic compounds. PA and PG might participate a futile redox cycle, which mediated ROS production in M. aeruginosa. These results suggested DNA strands and cell membrane were two targets of ROS induced by PA, and oxidative damage was an important mechanism for the toxicity of PA against M. aeruginosa. (C) 2016 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Environmental Sciences ; Toxicology |
| 研究领域[WOS] | Environmental Sciences & Ecology ; Toxicology |
| 关键词[WOS] | REACTIVE OXYGEN ; MYRIOPHYLLUM-SPICATUM ; ASCORBIC-ACID ; DNA-DAMAGE ; POLYPHENOLIC ALLELOCHEMICALS ; SUBMERGED MACROPHYTES ; MOLECULAR MECHANISMS ; FLOW-CYTOMETRY ; CYANOBACTERIA ; ANTIOXIDANT |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000380867200051 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/28387]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_学位论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Henan Normal Univ, Coll Fisheries, Xinxiang 453007, Henan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, Zhiying,Zhang, Yongyuan,Gao, Yunni,et al. Effects of pyrogallic acid on Microcystis aeruginosa: oxidative stress related toxicity[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2016,132:413-419. |
| APA | Lu, Zhiying.,Zhang, Yongyuan.,Gao, Yunni.,Liu, Biyun.,Sun, Xuemei.,...&Wu, Zhenbin.(2016).Effects of pyrogallic acid on Microcystis aeruginosa: oxidative stress related toxicity.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,132,413-419. |
| MLA | Lu, Zhiying,et al."Effects of pyrogallic acid on Microcystis aeruginosa: oxidative stress related toxicity".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 132(2016):413-419. |
入库方式： OAI收割
来源：水生生物研究所
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