Deficiency of dietary niacin impaired gill immunity and antioxidant capacity, and changes its tight junction proteins via regulating NF-kappa B, TOR, Nrf2 and MLCK signaling pathways in young grass carp (Ctenopharyngodon idella)
文献类型：期刊论文
| 作者 | Li, Shun-Quan1; Feng, Lin1,2,3; Jiang, Wei-Dan1,2,3; Liu, Yang1,2,3; Jiang, Jun1,2,3; Wu, Pei1,2,3; Kuang, Sheng-Yao4; Tang, Ling4; Tang, Wu-Neng4; Zhang, Yong-An5 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2016-08-01 |
| 卷号 | 55页码:212-222 |
| 关键词 | Niacin deficiency Grass carp (Ctenopharyngodon idella) Immunity Tight junction protein Antioxidant system Gill |
| ISSN号 | 1050-4648 |
| 英文摘要 | To investigate the effects of dietary niacin on gill immunity, tight junction proteins, antioxidant system and related signaling molecules mRNA expression, young grass carp (Ctenopharyngodon idella) were fed six diets containing graded levels of niacin (3.95-55.01 mg/kg diet) for 8 weeks. The study indicated that niacin deficiency decreased lysozyme and acid phosphatase activities, and complement 3 content, and caused oxidative damage that might be partly due to the decreased copper, zinc superoxide dismutase, catalase, glutathione reductase, glutathione peroxidase and glutathione-S-transferase activities and reduced glutathione content in fish gills (P < 0.05). Moreover, the relative mRNA levels of antimicrobial peptides (liver expressed antimicrobial peptide 2 and Hepcidin), anti-inflammatory cytokines (interleukin 10 and transforming growth factor On tight junction proteins (Occludin, zonula occludens 1, Claudin-15 and -3), signaling molecules (inhibitor of kappa B alpha (I kappa B alpha), target of rapamycin (TOR), ribosomal protein S6 kinase 1 (S6K1) and NF-E2-related factor 2 (Nrf2)) and antioxidant enzymes were significantly decreased (P < 0.05) in niacin-deficient diet group. Conversely, the mRNA levels of pro-inflammatory cytokines (tumor necrosis factor alpha, interleukin 8, interferon gamma 2, and interleukin 1 beta), signaling molecules (nuclear factor kappa B p65, I kappa B kinase alpha, I kappa B kinase 13, I kappa B kinase gamma, Kelch-like-ECH-associated protein 1b, myosin light chain kinase and p38 mitogen-activated protein kinase (p38 MAPK) were significantly increased (P < 0.05) in fish gills fed niacin-deficient diet. Interestingly, the varying niacin levels of 3.95-55.01 mg/kg diet had no effect on the mRNA level of Kelch-like-ECH-associated protein la, Claudin-c and -12 in fish gills (P > 0.05). In conclusion, niacin deficiency decreased gill immunity, impaired, gill antioxidant system, as well as regulated mRNA expression of gill tight junction proteins and related signaling molecules of fish. (C) 2016 Published by Elsevier Ltd. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 关键词[WOS] | INTESTINAL ENZYME-ACTIVITIES ; MESSENGER-RNA EXPRESSION ; SALMON SALMO-SALAR ; ATLANTIC SALMON ; GENE-EXPRESSION ; JUVENILE JIAN ; VAR. JIAN ; INFLAMMATORY RESPONSE ; GROWTH-PERFORMANCE ; GRADED-LEVELS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000381537000021 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/28351]  |
| 专题 | 水生生物研究所_水生生物多样性与资源保护研究中心_学位论文 |
| 作者单位 | 1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Peoples R China 2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Chengdu 611130, Peoples R China 3.Sichuan Agr Univ, China Minist Educ, Key Lab Anim Dis Resistance Nutr, Chengdu 611130, Peoples R China 4.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 610066, Peoples R China 5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Shun-Quan,Feng, Lin,Jiang, Wei-Dan,et al. Deficiency of dietary niacin impaired gill immunity and antioxidant capacity, and changes its tight junction proteins via regulating NF-kappa B, TOR, Nrf2 and MLCK signaling pathways in young grass carp (Ctenopharyngodon idella)[J]. FISH & SHELLFISH IMMUNOLOGY,2016,55:212-222. |
| APA | Li, Shun-Quan.,Feng, Lin.,Jiang, Wei-Dan.,Liu, Yang.,Jiang, Jun.,...&Zhou, Xiao-Qiu.(2016).Deficiency of dietary niacin impaired gill immunity and antioxidant capacity, and changes its tight junction proteins via regulating NF-kappa B, TOR, Nrf2 and MLCK signaling pathways in young grass carp (Ctenopharyngodon idella).FISH & SHELLFISH IMMUNOLOGY,55,212-222. |
| MLA | Li, Shun-Quan,et al."Deficiency of dietary niacin impaired gill immunity and antioxidant capacity, and changes its tight junction proteins via regulating NF-kappa B, TOR, Nrf2 and MLCK signaling pathways in young grass carp (Ctenopharyngodon idella)".FISH & SHELLFISH IMMUNOLOGY 55(2016):212-222. |
入库方式： OAI收割
来源：水生生物研究所
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