Global Phosphoproteomic Analysis Reveals the Involvement of Phosphorylation in Aflatoxins Biosynthesis in the Pathogenic Fungus Aspergillus flavus
文献类型：期刊论文
| 作者 | Ren, Silin1,2; Yang, Mingkun3; Li, Yu1,2; Zhang, Feng1,2; Chen, Zhuo3; Zhang, Jia3; Yang, Guang1,2; Yue, Yuewei1,2; Li, Siting3; Ge, Feng3 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2016-09-26 |
| 卷号 | 6 |
| ISSN号 | 2045-2322 |
| 英文摘要 | Aspergillus flavus is a pathogenic fungus that produces toxic and carcinogenic aflatoxins and is the causative agent of aflatoxicosis. A growing body of evidence indicates that reversible phosphorylation plays important roles in regulating diverse functions in this pathogen. However, only a few phosphoproteins of this fungus have been identified, which hampers our understanding of the roles of phosphorylation in A. flavus. So we performed a global and site-specific phosphoproteomic analysis of A. flavus. A total of 598 high-confidence phosphorylation sites were identified in 283 phosphoproteins. The identified phosphoproteins were involved in various biological processes, including signal transduction and aflatoxins biosynthesis. Five identified phosphoproteins associated with MAPK signal transduction and aflatoxins biosynthesis were validated by immunoblotting using phospho-specific antibodies. Further functional studies revealed that phosphorylation of the MAP kinase kinase kinase Ste11 affected aflatoxins biosynthesis in A. flavus. Our data represent the results of the first global survey of protein phosphorylation in A. flavus and reveal previously unappreciated roles for phosphorylation in the regulation of aflatoxins production. The generated dataset can serve as an important resource for the functional analysis of protein phosphorylation in A. flavus and facilitate the elucidation of phosphorylated signaling networks in this pathogen. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | MAP KINASE PATHWAYS ; PROTEIN-PHOSPHORYLATION ; SECONDARY METABOLISM ; SIGNALING NETWORKS ; OXIDATIVE STRESS ; SCLEROTIAL PRODUCTION ; TRANSCRIPTION FACTOR ; AMINO-ACIDS ; IN-VIVO ; PARASITICUS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000384048200001 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/28354]  |
| 专题 | 水生生物研究所_水生生物多样性与资源保护研究中心_学位论文 |
| 作者单位 | 1.Fujian Agr & Forestry Univ, Key Lab Pathogen Fungi & Mycotoxins Fujian Prov, Fuzhou 350002, Peoples R China 2.Fujian Agr & Forestry Univ, Sch Life Sci, Fuzhou 350002, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ren, Silin,Yang, Mingkun,Li, Yu,et al. Global Phosphoproteomic Analysis Reveals the Involvement of Phosphorylation in Aflatoxins Biosynthesis in the Pathogenic Fungus Aspergillus flavus[J]. SCIENTIFIC REPORTS,2016,6. |
| APA | Ren, Silin.,Yang, Mingkun.,Li, Yu.,Zhang, Feng.,Chen, Zhuo.,...&Wang, Shihua.(2016).Global Phosphoproteomic Analysis Reveals the Involvement of Phosphorylation in Aflatoxins Biosynthesis in the Pathogenic Fungus Aspergillus flavus.SCIENTIFIC REPORTS,6. |
| MLA | Ren, Silin,et al."Global Phosphoproteomic Analysis Reveals the Involvement of Phosphorylation in Aflatoxins Biosynthesis in the Pathogenic Fungus Aspergillus flavus".SCIENTIFIC REPORTS 6(2016). |
入库方式： OAI收割
来源：水生生物研究所
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